High-resolution melting (HRM) analysis for the detection of single nucleotide polymorphisms in microRNA target sites
High-resolution melting (HRM) analysis for the detection of single nucleotide polymorphisms in microRNA target sites
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DOI：
10.1016/j.cca.2012.03.007
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发表时间：
2012-07-11
影响因子：
5
通讯作者：
Chang, Jan-Gowth
中科院分区：
文献类型：
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作者：
Lin, Pei-Chin;Liu, Ta-Chih;Chang, Jan-Gowth
背景：microRNAs（miRNAs）的功能依赖于miRNAs与信使RNA（mRNAs）3 'UTR中靶序列的结合。从而增强mRNA的降解。压抑自己的表达。miRNA靶序列中的单核苷酸多态性（SNP）可能影响或损害miRNA的结合。方法：采用高分辨熔解曲线（HRM）分析3个miR-TS-SNPs（PLA 2G 2A. IL-16和NOD 2）在急性白血病中的作用。结果：HRM分析显示3个miR-TS-SNPs位点存在3种基因型（纯合型和杂合型）。所有3个miR-TS-SNPs的等位基因频率在正常个体和急性髓细胞性白血病患者中相似。结论：大规模病例对照研究miR-TS-SNPs的筛查有助于研究miR-TS-SNPs在人类疾病中的作用和发病机制。我们的研究表明，HRM分析可以成为miR-TS-SNPs研究的有效工具。(C)2012 Elsevier B. V.保留所有权利。
Background: The function of microRNAs (miRNAs) depends on the binding of miRNAs to their target sequences in the 3'UTR of messenger RNAs (mRNAs). which enhances the degradation of mRNAs and consequently. represses their expression. Single nucleotide polymorphisms (SNPs) in the miRNA target sequences may affect or impair the binding of miRNAs. Studies have shown that SNPs in miRNA target sites (miR-TS-SNPs) have a great influence on diverse biological functions, including pharmacogenomics and disease susceptibilities in human.Methods: High-resolution melting (HRM) analysis was applied for investigating the allele frequencies of 3 miR-TS-SNPs (PLA2G2A. IL-16, and NOD2) in acute leukemia. We also compared the genotypes of acute lymphoblastic leukemia patients at initial diagnosis and complete remission.Results: HRM analysis revealed 3 genotypes (both homozygous and heterozygous) in the 3 miR-TS-SNPs. The allele frequencies of all 3 miR-TS-SNPs were similar in normal individuals and patients with acute myelogenous leukemia. Most patients with acute lymphoblastic leukemia had the same genotypes at initial diagnosis and complete remission.Conclusions: Large scale scanning of case-control studies for miR-TS-SNPs may contribute to the investigation of their roles and pathogenesis mechanisms in human diseases. Our study showed that HRM analysis can be an efficient tool for studies of miR-TS-SNPs. (C) 2012 Elsevier B.V. All rights reserved.