Evaluation of the utility of COI and ITS markers as tools for population genetic studies of temperate gorgonians
Evaluation of the utility of COI and ITS markers as tools for population genetic studies of temperate gorgonians
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DOI：
10.1016/j.jembe.2006.05.006
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发表时间：
2006-09-05
影响因子：
2
通讯作者：
Aurelle, D.
中科院分区：
文献类型：
--
作者：
Calderon, I.;Garrabou, J.;Aurelle, D.
本研究的目的是分析线粒体COI和核ITS 2两个分子标记的多态性，以确定地中海柳珊瑚群体的遗传结构。分析了4种柳橙属植物、cavoliniella、singulunicella、Paramuricea clavata和Corallium rubrum的COI，而只分析了cavolinie的ITS。这些物种最近受到了可能与气候变化有关的大规模死亡事件的影响。在这些强烈干扰的背景下，关于受影响种群遗传结构的可靠数据对于提出这些物种的管理和保护策略至关重要。设计了一个包含不同空间尺度（从十分之一到数百公里）的分层抽样计划来测试这些标记。这两种标记都未能证明这些种群的任何遗传结构。讨论了这些标记的进化特征和应用，并提出了未来的研究方向。(c) 2006 Elsevier B.V.版权所有
The aim of this study was to analyse the polymorphism of two molecular markers, mitochondrial COI and nuclear ITS 2, to determine the genetic structure of Mediterranean gorgonian populations. Four gorgonians, Eunicella cavolinii, Eunicella singularis, Paramuricea clavata and Corallium rubrum were analysed for COI, while only E. cavolinii was analysed for ITS. These species have been recently affected by mass mortality events probably linked to climate change. In the context of these strong disturbances, reliable data on the genetic structure of affected populations could be critical in order to propose management and conservation strategies for these species. A hierarchical sampling plan encompassing different spatial scales (from tenths to hundreds of kilometres) was designed to test these markers. Both markers failed to make evident any genetic structure of these populations. The evolutionary features and utility of these markers are critically discussed and future research directions are proposed. (c) 2006 Elsevier B.V. All rights reserved.