Existence of a bovine LINE repetitive insert that appears in the cDNA of bovine protein BCNT in ruminant, but not in human, genomes.
Existence of a bovine LINE repetitive insert that appears in the cDNA of bovine protein BCNT in ruminant, but not in human, genomes.
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牛 LINE 重复插入片段存在于反刍动物基因组中的牛蛋白 BCNT 的 cDNA 中，但在人类基因组中则没有。
DOI：
10.1016/s0378-1119(98)00136-x
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发表时间：
1998
期刊：
影响因子：
3.5
通讯作者：
S. Iwashita
中科院分区：
文献类型：
--
作者：
I. Takahashi;T. Nobukuni;H. Ohmori;M. Kobayashi;S. Tanaka;K. Ohshima;N. Okada;T. Masui;K. Hashimoto;S. Iwashita
BCNT是一种新的蛋白质，最初从牛脑中分离出来，以Bucentaur命名，它包含一个从部分牛线重复序列(Bov-B线)翻译而来的内部部分。已分离到与牛bcnt(Bbcnt)高度同源的人的cDNA，但不包含与Bov-B线插入序列(Nobukuni，T.，Kobayashi，M.，Omori，A.，Ichinose，S.，Iwanaga，T.，Takahashi，I.，Hashimoto，K.，Hattori，S.，Kaibuhi，K.，Miyata，Y.，Masui，T.，Iwashita，S.，1997)相似的序列。在一种新的牛蛋白中表达了对应于280个氨基酸的Alu连接的重复序列，但在其人类同源物中没有表达。J.Biol.化学。272、2801-2807)。在这项研究中，我们进行了聚合酶链式反应分析，以调查这种BoV-B线插入是否存在于其他动物的bcnt同源基因和人类BCNT(HBCNT)基因的基因组序列中。结果表明，Bov-B插入序列存在于绵羊、山羊、中轴鹿和鼠鹿(Chevrotin)的bcnt同源物的基因组序列中，即在瘤胃动物中存在，但在猪和人中没有。对Bov-B株插入物周围的bbcnt基因组序列的分析表明，插入的Bov-B株序列有很大一部分包含在外显子中；紧随其后的是与BoV-B株3‘端内含子高度同源的54个核苷酸序列。HBCNT基因由7个外显子和6个内含子组成，其中与bbcnt基因组中Bov-B序列插入位点对应的内含子长度为16.5kb，没有与BoV-B序列相似的序列。根据这些结果，Bov-B株插入片段很可能来源于一个长的Bov-B株重复序列，该重复序列转座到瘤胃动物的祖先bcnt基因上，并通过转座序列中的新剪接位点改造为新的外显子。
A novel protein, BCNT, originally isolated from bovine brain and named after Bucentaur, contains an internal portion that is translated from part of bovine LINE repetitive sequence (Bov-B LINE). Human cDNA highly homologous to the bovine bcnt (bbcnt) cDNA has been isolated but does not contain a sequence similar to the Bov-B LINE insert (Nobukuni, T., Kobayashi, M., Omori, A., Ichinose, S., Iwanaga, T., Takahashi, I., Hashimoto, K., Hattori, S., Kaibuchi, K., Miyata, Y., Masui, T., Iwashita, S., 1997. An Alu-linked repetitive sequence corresponding to 280 amino acids is expressed in a novel bovine protein, but not in its human homologue. J. Biol. Chem. 272, 2801–2807). In this study, we conducted a polymerase chain reaction analysis to investigate whether such a Bov-B LINE insert is present in bcnt orthologs in other animals and in the genomic sequence of the human BCNT (hBCNT) gene. The results indicate that the Bov-B LINE insert is present in the genomic sequences of bcnt orthologs from sheep, goats, axis deer, and mouse deer (chevrotin), that is in Ruminantia, but not in pigs or human. Analysis of the bbcnt genomic sequence around the Bov-B LINE insert revealed a large part of the inserted Bov-B LINE sequence to be included in an exon; this is followed by a 54-nucleotide sequence that is highly homologous to Bov-B LINE in the 3′-side intron. The hBCNT gene was isolated and found to consist of seven exons and six introns, among which the intron corresponding to the Bov-B LINE insertion site in the bbcnt genome is 16.5kb in length with no sequence similar to Bov-B LINE. Based on these results, it seems likely that the Bov-B LINE insert is derived from a long Bov-B LINE repetitive sequence transposed to an ancestral bcnt gene in Ruminantia and reformed as a new exon through new splicing sites in the transposed sequence.
DOI：
--
发表时间：
1991
期刊：
Advances in second messenger and phosphoprotein research
影响因子：
--
作者：
P. T. Tuazon;J. Traugh
通讯作者：
P. T. Tuazon;J. Traugh