Social network architecture of human immune cells unveiled by quantitative proteomics
Social network architecture of human immune cells unveiled by quantitative proteomics
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DOI：
10.1038/ni.3693
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发表时间：
2017-05-01
影响因子：
30.5
通讯作者：
Meissner, Felix
中科院分区：
文献类型：
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作者：
Rieckmann, Jan C.;Geiger, Roger;Meissner, Felix
免疫系统在众多细胞类型之间的动态相互作用方面是独特的。然而，关于免疫细胞如何交流以抵御疾病的全系统观点尚未建立。我们应用基于高分辨率质谱的蛋白质组学来表征28个处于稳定和活化状态的原代人类造血细胞群体，其深度总计> 10，000个蛋白质。蛋白质拷贝数揭示了配体和受体表达的免疫细胞的特化，从而连接不同的免疫功能。通过整合总蛋白质组和分泌蛋白质组，我们发现了基本的细胞间通讯结构和以前未知的细胞类型之间的连接。我们的公开访问（http：//www.immprot.org/）的蛋白质组资源提供了一个框架，协调细胞的相互作用和参考改变与病理学相关的通信。
The immune system is unique in its dynamic interplay between numerous cell types. However, a system-wide view of how immune cells communicate to protect against disease has not yet been established. We applied high-resolution mass-spectrometry-based proteomics to characterize 28 primary human hematopoietic cell populations in steady and activated states at a depth of >10,000 proteins in total. Protein copy numbers revealed a specialization of immune cells for ligand and receptor expression, thereby connecting distinct immune functions. By integrating total and secreted proteomes, we discovered fundamental intercellular communication structures and previously unknown connections between cell types. Our publicly accessible (http://www.immprot.org/) proteomic resource provides a framework for the orchestration of cellular interplay and a reference for altered communication associated with pathology.