Studies on polynucleotides. LXXIX. Yeast phenylalanine transfer ribonucleic acid: products obtained by degradation with pancreatic ribonuclease.
Studies on polynucleotides. LXXIX. Yeast phenylalanine transfer ribonucleic acid: products obtained by degradation with pancreatic ribonuclease.
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多核苷酸的研究。
DOI：
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发表时间：
1968
影响因子：
4.8
通讯作者：
A. Stuart
中科院分区：
文献类型：
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作者：
U. RajBhandary;R. Faulkner;A. Stuart
摘要 用胰腺核糖核酸酶降解酵母纯化的苯丙氨酸转移RNA（tRNA），并对所得片段进行分离和鉴定。 3'-末端核苷是胞苷，5'-末端二核苷酸是pGpCp。存在 14 种次要核苷，包括 7-甲基鸟苷、2'-O-甲基胞苷和身份尚未确定的核苷。涉及一些次要核苷、N2-二甲基-GpCp、GpTp 和 ApGp-二氢-Up 的序列对于许多其他 tRNA 来说是常见的。产生的最大寡核苷酸是具有序列 GpGpGpApGpAp-GpCp 的八核苷酸。
Abstract Purified phenylalanine transfer RNA (tRNA) from yeast has been degraded with pancreatic ribonuclease, and the fragments obtained have been separated and identified. The 3'-terminal nucleoside is cytidine, and the 5'-terminal dinucleotide is pGpCp. Fourteen minor nucleosides are present, including 7-methylguanosine, 2'-O-methylcytidine, and a nucleoside the identity of which is not established. The sequences involving some of the minor nucleosides, N2-dimethyl-GpCp, GpTp, and ApGp-dihydro-Up, are common to many other tRNAs. The largest oligonucleotide produced is an octanucleotide with the sequence GpGpGpApGpAp-GpCp.