Different response of satellite cells in the kinetics of myogenic regulatory factors and ultrastructural pathology after Trichinella spiralis and T-pseudospiralis infection
Different response of satellite cells in the kinetics of myogenic regulatory factors and ultrastructural pathology after Trichinella spiralis and T-pseudospiralis infection
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DOI：
10.1017/s0031182001007958
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发表时间：
2001-07-01
期刊：
影响因子：
2.4
通讯作者：
Takahashi, Y
中科院分区：
文献类型：
--
作者：
Wu, Z;Matsuo, A;Takahashi, Y
感染胞内寄生线虫，旋毛虫，导致肌肉细胞的严重损伤，其次是卫星细胞的激活和增殖。损伤后不久的修复过程与机械损伤后的组织病理学相似。相似性也适用于肌生成调节因子（MyoD，肌生成素和MRF4）的表达动力学。不同之处在于肌肉细胞感染T.螺旋体转化为一种独特的细胞，在寄生虫学上称为营养细胞，并且增殖的卫星细胞不分化为肌细胞，而是分化为营养细胞（误分化）。因此，营养细胞是转化的感染肌细胞和分化不良的卫星细胞的融合体。感染另一种旋毛虫，T。pseudospiralis也引起细胞损伤，但更广泛地涉及感染的肌细胞的整个长度，因为没有形成隔膜以最小化受影响的区域。因此，大量卫星细胞被激活并增殖。肌细胞生成调节因子如MyoD和myogenin的激活时间比T.螺旋体感染感染的肌细胞转化为营养细胞，其胞质内具有广泛的滑面内质网。卫星细胞分化为营养细胞，其细胞质无定形，缺乏明显的细胞器。这两种细胞质没有融合，因为迄今为止检查。因此，T. spiralis和T. pseudospiralis引起的卫星细胞的分化不良，但在不同的方式。
Infection of an intracellular parasitic nematode, Trichinella spiralis, resulted in severe damage in muscle cells which was followed by activation and proliferation of satellite cells. The repairing process, shortly after the damage, histopathologically resembled those seen after mechanical injury. Resemblance was also true for kinetics of expression of myogenic regulatory factors (MyoD, myogenin and MRF4). The difference resided in the next step where the muscle cell infected with T. spiralis transformed to a unique cell which is parasitologically known as the nurse cell, and the proliferated satellite cells did not differentiate to the muscle cell but to the nurse cell (misdifferentiation). Thus the nurse cell was a fusion of the transformed infected muscle cell and misdifferentiated satellite cells. Infection with another species of Trichinella, T. pseudospiralis, also caused cell damage, but more extensively involving the entire length of the infected muscle cells because no septum was formed to minimize the affected area. Therefore, a large number of satellite cells were activated and proliferated. The myogenic regulatory factors such as MyoD and myogenin were activated for a longer period than in the case with T. spiralis infection. The infected muscle cell transformed to the nurse cell, whose cytoplasm was characterized by extensive smooth endoplasmic reticulum. Satellite cells misdifferentiated to the nurse cell, whose cytoplasm was amorphous, void of distinct cell organelles. The two kinds of cytoplasm did not fuse as examined thus far. Thus infection with T. spiralis and T. pseudospiralis caused misdifferentiation of satellite cells, but in a different way.