The influence of cell mechanics, cell-cell interactions, and proliferation on epithelial packing
The influence of cell mechanics, cell-cell interactions, and proliferation on epithelial packing
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DOI：
10.1016/j.cub.2007.11.049
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发表时间：
2007-12-18
期刊：
影响因子：
9.2
通讯作者：
Juelicher, Frank
中科院分区：
文献类型：
--
作者：
Farhadifar, Reza;Roeper, Jens-Christian;Juelicher, Frank
背景：上皮连接网络呈现以不同细胞形状、相邻细胞数量分布和面积为特征的堆积几何形状。特定包装几何形状的发展受到严格控制;在果蝇翅膀上皮中，细胞在毛发形成前不久从不规则阵列转化为六边形阵列。包装的几何形状是由发展机制，可能控制的生物物理性质的细胞和它们的interactions.Results：要了解物理细胞特性和增殖如何确定细胞包装的几何形状，我们使用的顶点模型的上皮交界网络中，细胞包装的几何形状对应于稳定和静止的网络配置。该模型考虑了细胞弹性和皮质收缩和粘附所产生的连接力。通过数值模拟增殖，我们产生不同的网络形态，取决于物理参数。这些网络在多边形类分布、细胞面积变化以及生长过程中T1和T2过渡的速率方面有所不同。将理论结果与观察到的细胞形态进行比较，揭示了计算出的网络形态与观察到的网络形态相匹配的参数空间区域。我们独立估计参数值通过量化网络变形所造成的激光烧蚀个别cell boundaries.Conclusions：顶点模型占定性和定量观察到的包装几何形状在翼盘和其响应扰动激光烧蚀。上皮堆积几何形状是细胞物理性质和增殖的无序影响的结果。网络生长过程中T2转换的发生表明，从增殖的椎间盘上皮细胞的消除可能是交界力平衡的结果。
Background: Epithelial junctional networks assume packing geometries characterized by different cell shapes, neighbor number distributions and areas. The development of specific packing geometries is tightly controlled; in the Drosophila wing epithelium, cells convert from an irregular to a hexagonal array shortly before hair formation. Packing geometry is determined by developmental mechanisms that likely control the biophysical properties of cells and their interactions.Results: To understand how physical cellular properties and proliferation determine cell-packing geometries, we use a vertex model for the epithelial junctional network in which cell packing geometries correspond to stable and stationary network configurations. The model takes into account cell elasticity and junctional forces arising from cortical contractility and adhesion. By numerically simulating proliferation, we generate different network morphologies that depend on physical parameters. These networks differ in polygon class distribution, cell area variation, and the rate of T1 and T2 transitions during growth. Comparing theoretical results to observed cell morphologies reveals regions of parameter space where calculated network morphologies match observed ones. We independently estimate parameter values by quantifying network deformations caused by laser ablating individual cell boundaries.Conclusions: The vertex model accounts qualitatively and quantitatively for the observed packing geometry in the wing disc and its response to perturbation by laser ablation. Epithelial packing geometry is a consequence of both physical cellular properties and the disordering influence of proliferation. The occurrence of T2 transitions during network growth suggests that elimination of cells from the proliferating disc epithelium may be the result of junctional force balances.