Magnetophoretic mobilities correlate to antibody binding capacities
Magnetophoretic mobilities correlate to antibody binding capacities
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DOI：
10.1002/1097-0320(20000801)40:4
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发表时间：
2000-08-01
期刊：
影响因子：
--
通讯作者：
Zborowski, M
中科院分区：
文献类型：
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作者：
McCloskey, KE;Chalmers, JJ;Zborowski, M
方法：采用免疫磁性标记和细胞跟踪测速(CTV)技术，定量检测细胞表面抗原的表达水平。结果：抗CD2异硫氰酸荧光素(FITC)抗体和抗FITC-MACs顺磁性纳米粒免疫磁性标记了QSC微球。磁致迁移率被定义为标记细胞或微球的磁感应速度除以磁致驱动力，与磁能密度梯度成正比。讨论：利用计算机成像和处理技术，通过显微记录和计算免疫磁化标记QSC微球在几乎恒定的磁能梯度下的速度来完成迁移率的测量。得到了免疫磁性标记微球的磁电迁移率与其抗体结合容量(ABC)之间的校正曲线。结论：对于ABC<30,000的微球，磁致迁移率与ABC之间存在线性关系，与理论结果一致。获得的数学关系和QSC标准化曲线允许确定类似免疫磁性标记细胞上的表面抗原的数量。(C)2000 Wiley-Liss，Inc.
Methods: A methodology and a mathematical theory have been developed, which allow quantitation of the expression levels of cellular surface antigens using immunomagnetic labels and cell tracking velocimetry (CTV) technology.Results: Quantum Simply Cellular (QSC) microbeads were immunomagnetically labeled with anti-CD2 fluorescein isothiocyanate (FITC) antibodies and anti-FITC MACS paramagnetic nanoparticles. Magnetophoretic mobility has been defined as the magnetically induced velocity of the labeled cell or microbead divided by the magnetophoretic driving force, proportional to the magnetic energy density gradient.Discussion: Using computer imaging and processing technology, the mobility measurements were accomplished by microscopically recording and calculating the velocity of immunomagnetically labeled QSC microbeads in a nearly constant magnetic energy gradient. A calibration curve correlating the measured magnetophoretic mobility of the immunomagnetically labeled microbeads to their antibody binding capacities (ABC) has been obtained.Conclusion: The results, in agreement with theory, indicate a Linear relationship between magnetophoretic mobility and ABC for microbeads with less than 30,000 ABC. The mathematical relationships and QSC standardization curve obtained allow determination of the number of surface antigens on similarly immunomagnetically labeled cells. (C) 2000 Wiley-Liss, Inc.