IFN-γ modulates Ly-49 receptors on NK cells in IFN-γ-induced pregnancy failure.
IFN-γ modulates Ly-49 receptors on NK cells in IFN-γ-induced pregnancy failure.
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DOI：
10.1038/srep18159
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发表时间：
2015-12-11
影响因子：
4.6
通讯作者：
Peng JP
中科院分区：
文献类型：
--
作者：
Li ZY;Song ZH;Meng CY;Yang DD;Yang Y;Peng JP
我们先前已经表明，干扰素γ（IFN-γ）通过调节CX 3CL 1诱导异常的CD 49 b+自然杀伤（NK）细胞募集，并最终引起胎儿丢失。在这项研究中，我们表明，IFN-γ也调节妊娠失败期间NK细胞上的Ly-49受体。IFN-γ处理组子宫中Ly-49 A+和Ly-49 G2 + NK细胞的百分比显著低于对照组中观察到的那些。IFN-γ处理组子宫NK细胞Ly-49 A和Ly-49 G2表达的荧光强度中位数（MFI）显著低于对照组。使用分离的脾白细胞，我们进一步发现IFN-γ在体外显著降低Ly-49 A + NK细胞的百分比。但CX 3CL 1不参与Ly-49受体的调节，且脾白细胞CX 3CR 1的表达不受IFN-γ的调节。总的来说，我们的数据表明，IFN-γ可以调节NK细胞上的Ly-49受体，这一过程可能在IFN-γ诱导的妊娠失败中发挥作用。因此，我们提供了一条新的证据，将IFN-γ的有害作用与其在妊娠失败期间调节NK细胞Ly-49受体的作用相关联。
We have previously shown that interferon gamma (IFN-γ) induces aberrant CD49b+ natural killer (NK) cell recruitment by regulating CX3CL1 and eventually provokes foetal loss. In this study, we show that IFN-γ also modulates Ly-49 receptors on NK cells during pregnancy failure. The percentages of Ly-49A+ and Ly-49G2+ NK cells in the uteri of the IFN-γ-treated group were significantly lower than those observed in the control group. Moreover, the median fluorescence intensity (MFI) values of Ly-49A and Ly-49G2 expression on NK cells in the uteri of the IFN-γ-treated group were significantly lower than those of the control group. Using isolated spleen leucocytes, we further found that IFN-γ significantly reduced the percentage of Ly-49A+ NK cells in vitro. However, CX3CL1 was not involved in the modulation of Ly-49 receptors, and the expression of CX3CR1 was not regulated by IFN-γ in spleen leucocytes. Collectively, our data indicate that IFN-γ can modulate Ly-49 receptors on NK cells and this process may play a role in IFN-γ-induced pregnancy failure. Thus, we provide a new line of evidence correlating the deleterious effects of IFN-γ with its role in regulating NK cell Ly-49 receptors during pregnancy failure.