Simplification of the end-alveolar carbon monoxide technique to assess erythrocyte survival
Simplification of the end-alveolar carbon monoxide technique to assess erythrocyte survival
复制标题
DOI：
10.1016/s0022-2143(03)00086-6
复制
发表时间：
2003-07-01
期刊：
影响因子：
--
通讯作者：
Levitt, MD
中科院分区：
文献类型：
--
作者：
Furne, JK;Springfield, JR;Levitt, MD
我们描述了一种简单、快速地确定红细胞(RBC)寿命的方法，该方法基于测量经大气一氧化碳(CO)校正后的肺泡一氧化碳(CO)浓度，该浓度是用模拟人体与CO平衡的装置测定的。这项研究旨在确定是否可以用平衡仪设备直接测量环境中的CO来代替采样。在我们的医疗中心获得的几周的大气采样表明，CO浓度足够恒定，以至于在呼气收集时的一次环境CO测量充分校正了大气CO。对志愿者的其他研究表明，受试者家中过夜的一氧化碳浓度也保持相对恒定，这表明可以从受试者早上收集的肺泡一氧化碳浓度和家中大气样本中获得对红细胞寿命的准确评估。使用这项技术，我们发现40名健康志愿者的红细胞寿命平均为122+/-23天，这一数值与复杂队列标记方法得出的值相当。在这些个体中，红细胞寿命和网织红细胞计数之间没有明显的相关性。这项简单的技术在4名接受利巴韦林和干扰素治疗丙型肝炎的受试者中证明了检测到红细胞周转增加的能力，据报道，这种治疗方法可以缩短红细胞寿命。对这四个受试者在其家庭环境中收集的样本中一氧化碳的测量表明，每个受试者的红细胞寿命都缩短了(30-69天)。
We have described a simple, rapid method for determining red blood cell (RBC) life span based on measurement of alveolar carbon monoxide (CO) concentration corrected for atmospheric CO as determined with a device that simulates the body's equilibration with CO. This study was designed to determine whether direct measurements of ambient CO can be substituted for sampling with the equilibrator device. Atmospheric samplings obtained in our medical center over a period of weeks indicated that CO concentration was sufficiently constant that a single ambient CO measurement at the time of breath collection adequately corrected for atmospheric CO. Additional studies with volunteers showed that overnight CO concentrations in the subjects' home environments also remained relatively constant, suggesting that accurate assessment of RBC life span can be obtained from alveolar CO concentrations and home atmospheric samples collected on a subject's arising in the morning. Using this technique, we found that the RBC life span of 40 healthy volunteers averaged 122 +/- 23 days, a value comparable to that obtained with complex cohort labeling methods. No significant correlation was observed between RBC life span and reticulocyte count in these individuals. The ability of this simple technique to detect increased RBC turnover was demonstrated in 4 subjects being treated with ribavirin and interferon for hepatitis C, a treatment reported to shorten RBC life span. Measurement of CO in samples collected by these four subjects in their home environments indicated that each had a shortened RBC life span (range 30-69 days).