FURIN IS IMPORTANT BUT NOT ESSENTIAL FOR THE PROTEOLYTIC MATURATION OF GP160 OF HIV-1
FURIN IS IMPORTANT BUT NOT ESSENTIAL FOR THE PROTEOLYTIC MATURATION OF GP160 OF HIV-1
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DOI：
10.1016/0014-5793(95)00447-h
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发表时间：
1995-05-22
期刊：
影响因子：
3.5
通讯作者：
LEPPLA, SH
中科院分区：
文献类型：
--
作者：
GU, ML;RAPPAPORT, J;LEPPLA, SH
HIV的包膜糖蛋白是病毒感染性所必需的。前体包膜糖蛋白gp 160的蛋白水解导致gp 120和gp 41的形成。切割发生在序列Arg-Glu-Lys-Arg之后。预期该序列是细胞蛋白酶弗林蛋白酶的底物。我们研究是否弗林蛋白酶是负责切割的gp 160通过使用弗林蛋白酶缺陷的CHO细胞系和相同的细胞系转染弗林蛋白酶cDNA。从病毒传播试验中获得的数据表明，弗林蛋白酶增加病毒的感染性，但不是必不可少的成熟的gp 160，这意味着其他前蛋白加工酶也认识到这个假定的弗林蛋白酶切割位点。
The envelope glycoproteins of HIV are required for viral infectivity. Proteolgsis of the Precursor envelope glycoprotein gp160 results in the formation of gp120 acid gp41. Cleavage occurs after the sequence Arg-Glu-Lys-Arg. This sequence is expected to be a substrate for the cellular protease furin. We examined whether furin is responsible for cleavage of gp160 by using a furin-deficient CHO cell line and the same cell line transfected with furin cDNA. Data obtained from viral transmission assays suggested that furin increased viral infectivity but was not essential for the maturation of gp160, implying that other proprotein processing enzymes also recognize this putative furin cleavage site.