Urea sensitizes mIMCD3 cells to heat shock-induced apoptosis: protection by NaCl.
Urea sensitizes mIMCD3 cells to heat shock-induced apoptosis: protection by NaCl.
复制标题
尿素使 mIMCD3 细胞对热休克诱导的细胞凋亡敏感：氯化钠的保护。
DOI：
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Germain Rousselet
中科院分区：
文献类型：
--
作者：
Chantal Colmont;Stéphanie Michelet;Dominique Guivarc'h;Germain Rousselet
Urea, with NaCl, constitutes the osmotic gradient that allows water reabsorption in mammalian kidneys. Because NaCl induces heat shock proteins, we tested the responses to heat shock of mIMCD3 cells adapted to permissive urea and/or NaCl concentrations. We found that heat-induced cell death was stronger after adaptation to 250 mM urea. This effect was reversible, dose dependent, and, interestingly, blunted by 125 mM NaCl. Moreover, we have shown that urea-adapted cells engaged in an apoptotic pathway upon heat shock, as shown by DNA laddering. This sensitization is not linked to a defect in the heat shock response, because the induction of HSP70 was similar in isotonic and urea-adapted cells. Moreover, it is not linked to the presence of urea inside cells, because washing urea away did not restore heat resistance and because applying urea and heat shock at the same time did not lead to heat sensitivity. Together, these results suggest that urea modifies the heat shock response, leading to facilitated apoptosis.
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影响因子：
--
作者：
RAUCHMAN, MI;NIGAM, SK;GULLANS, SR
通讯作者：
GULLANS, SR
DOI：
10.1152/ajprenal.1999.276.5.f786
发表时间：
1999-05
期刊：
American journal of physiology. Renal physiology
影响因子：
--
作者：
Z Zhang-;X. Yang;D. Cohen
通讯作者：
Z Zhang-;X. Yang;D. Cohen
DOI：
10.1152/ajprenal.2000.278.2.f209
发表时间：
2000-02-01
影响因子：
4.2
作者：
Michea, L;Ferguson, DR;Burg, MB
通讯作者：
Burg, MB
影响因子：
15.9
作者：
Cohen, DM;Gullans, SR;Chin, WW
通讯作者：
Chin, WW
DOI：
10.1073/pnas.93.20.11242
发表时间：
1996-10-01
影响因子：
11.1
作者：
Cohen, DM
通讯作者：
Cohen, DM