5-hydroxytryptophan as a new intrinsic probe for investigating protein-DNA interactions by analytical ultracentrifugation. Study of the effect of DNA on self-assembly of the bacteriophage lambda cI repressor.
5-hydroxytryptophan as a new intrinsic probe for investigating protein-DNA interactions by analytical ultracentrifugation. Study of the effect of DNA on self-assembly of the bacteriophage lambda cI repressor.
复制标题
5-羟基色氨酸作为一种新的内在探针，用于通过分析超速离心研究蛋白质-DNA 相互作用。
DOI：
10.1021/bi00061a003
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发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Ross,JB
中科院分区：
文献类型：
--
作者：
Laue,TM;Senear,DF;Eaton,S;Ross,JB
方法：蛋白质和操作符DNA。野生型X - cl和5-OHTrp-X - cl的表达和分离按照其他地方的描述进行（Ross等，in press）。抑制因子在Superdex 75 HR 10/30 FPLC （Pharmacia, Inc）柱上进一步纯化。蛋白质浓度由色氨酸（5500 M-1 cm-1）和酪氨酸（1200 M-1 cm-1）的平均280 nm消光系数估计。
METHODSProteins and Operator DNA. Expression and isolation of wild-type X cl and 5-OHTrp-X cl were performed as described elsewhere (Ross et al., in press). Repressor was further purified by chromatography on a Superdex 75 HR 10/30 FPLC (Pharmacia, Inc.) column. Protein concentration was esti-mated from average 280-nmextinction coefficients for tryptophan (5500 M_1 cm-1) and tyrosine (1200 M-1 cm-1)