New Insights into the Functions of Transcription Factors that Bind the RNA Polymerase Secondary Channel.
New Insights into the Functions of Transcription Factors that Bind the RNA Polymerase Secondary Channel.
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DOI：
10.3390/biom5031195
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发表时间：
2015-06-25
期刊：
影响因子：
5.5
通讯作者：
Yuzenkova Y
中科院分区：
文献类型：
--
作者：
Zenkin N;Yuzenkova Y
转录延伸在几个不同的水平进行调节，包括由各种辅助转录延伸因子控制。这些因子中的一个独特的组与RNA聚合酶二级通道相互作用，二级通道是通向其活性中心的酶表面的开口。尽管研究了几年，这些因素的活动和在体内的作用仍然不清楚。本文就细菌次级通道结合因子的功能研究进展作一综述。特别是，我们强调了令人惊讶的作用，全球调节DksA在保真度的RNA合成和解决RNA聚合酶交通堵塞的GRE因子。这些发现表明转录保真度与转录和复制机制的碰撞之间存在潜在联系。
Transcription elongation is regulated at several different levels, including control by various accessory transcription elongation factors. A distinct group of these factors interacts with the RNA polymerase secondary channel, an opening at the enzyme surface that leads to its active center. Despite investigation for several years, the activities and in vivo roles of some of these factors remain obscure. Here, we review the recent progress in understanding the functions of the secondary channel binding factors in bacteria. In particular, we highlight the surprising role of global regulator DksA in fidelity of RNA synthesis and the resolution of RNA polymerase traffic jams by the Gre factor. These findings indicate a potential link between transcription fidelity and collisions of the transcription and replication machineries.