Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans
Characterization of a recombinant endo-type alginate lyase (Alg7D) from Saccharophagus degradans
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DOI：
10.1007/s10529-012-0876-9
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发表时间：
2012-06-01
影响因子：
2.7
通讯作者：
Kim, Kyoung Heon
中科院分区：
文献类型：
--
作者：
Kim, Hee Taek;Ko, Hyeok-Jin;Kim, Kyoung Heon
在大肠杆菌中高效表达了降解糖酵母的藻酸裂解酶基因，该基因编码一个属于多糖裂解酶-7家族的海藻酸裂解酶。对重组海藻酸7D进行了表征。该酶通过β-消除将海藻酸盐内切解聚为聚合度为2-5的低聚海藻酸盐。当温度为50A，pH为7时，酶活性最高，在Na+存在时，酶活性略有升高。K(M)、V(Max)、k(CAT)和k(CAT)/K(M)分别为：3 mg ml(-1)、6.2U mg(-1)、1.9×10(-2)S(-1)和6.3×10(-3)mg(-3)ml S(-1)。
A gene, alg7D, from Saccharophagus degradans, coding for a putative alginate lyase belonging to the family of polysaccharide lyase-7, was overexpressed in Escherichia coli. The properties of the recombinant Alg7D were characterized. The enzyme endolytically depolymerized alginate by beta-elimination into oligo-alginates with degrees of polymerization of 2-5. Its activity was maximal at 50A degrees C and pH 7 and was slightly increased in the presence of Na+. The K (M) , V (max) , k (cat) , and k (cat) /K (M) values were: 3 mg ml(-1), 6.2 U mg(-1), 1.9 x 10(-2) s(-1), and 6.3 x 10(-3) mg(-1) ml s(-1), respectively.