IDENTIFICATION AND LOCALIZATION OF SYNAPTOPHYSIN, AN INTEGRAL MEMBRANE GLYCOPROTEIN OF MR 38,000 CHARACTERISTIC OF PRESYNAPTIC VESICLES
IDENTIFICATION AND LOCALIZATION OF SYNAPTOPHYSIN, AN INTEGRAL MEMBRANE GLYCOPROTEIN OF MR 38,000 CHARACTERISTIC OF PRESYNAPTIC VESICLES
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DOI：
10.1016/s0092-8674(85)80082-9
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
FRANKE, WW
中科院分区：
文献类型：
--
作者：
WIEDENMANN, B;FRANKE, WW
使用单克隆抗体 SY38，将分子量为 38,000 的多肽鉴定为突触前囊泡膜的特定成分。这种蛋白质呈酸性（等电点约为 pH 4.8）且糖基化，似乎是一种完整的膜蛋白，正如其用非离子去污剂 Triton X-100 溶解以及抗体 SY38 识别的表位位于这些囊泡的细胞质表面上的发现所表明的那样。它存在于牛脑、脊髓和视网膜神经元的突触前囊泡以及神经肌肉接头处。它也存在于肾上腺髓质中。它在不同脊椎动物物种中的出现表明其在进化过程中的稳定性。这种蛋白质被命名为突触素，它为突触前小泡膜提供了分子标记，可能参与突触小泡的形成和胞吐作用。
A polypeptide of MW 38,000 was identified as a specific component of the membrane of presynaptic vesicles, using the monoclonal antibody SY38. This protein, which is acidic (isoelectric at .apprx. pH 4.8) and glycosylated, appears to be an integral membrane protein, as suggested by its solubilization with the nonionic detergent Triton X-100 and the finding that the epitope recognized by antibody SY38 is located on the cytoplasmic surface of those vesicles. It is found in presynaptic vesicles of neurons of the [bovine] brain, spinal cord and retina as well as at neuromuscular junctions. It is also found in the adrenal medulla. Its occurrence in diverse vertebrate species indicates its stability during evolution. This protein, for which the name synaptophysin, is proposed, provides a molecular marker for the presynaptic vesicle membrane and may be involved in synpatic vesicle formation and exocytosis.