Crossfinder-assisted mapping of protein crosslinks formed by site-specifically incorporated crosslinkers
Crossfinder-assisted mapping of protein crosslinks formed by site-specifically incorporated crosslinkers
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DOI：
10.1093/bioinformatics/btv083
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发表时间：
2015-06-15
期刊：
影响因子：
5.8
通讯作者：
Mueller-Planitz, Felix
中科院分区：
文献类型：
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作者：
Mueller-Planitz, Felix
几十年来，蛋白质交联一直被用来获得关于蛋白质和蛋白质复合体的结构信息。然而，直到最近，随着高分辨率质谱学(MS)的出现，绘制参与交联键的氨基酸图谱才成为可能。在这里，我们介绍了Crossfinder，它自动搜索由LC-MS-MS数据中特定位点结合的交联氨基酸形成的交联键。
Protein crosslinking has been used for decades to derive structural information about proteins and protein complexes. Only recently, however, it became possible to map the amino acids involved in the crosslinks with the advent of high resolution mass spectrometry (MS). Here, we present Crossfinder, which automates the search for crosslinks formed by site-specifically incorporated crosslinking amino acids in LC-MS-MS data.