THE BULK OF THE PERIPHERAL B-CELL POOL IN MICE IS STABLE AND NOT RAPIDLY RENEWED FROM THE BONE-MARROW
THE BULK OF THE PERIPHERAL B-CELL POOL IN MICE IS STABLE AND NOT RAPIDLY RENEWED FROM THE BONE-MARROW
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DOI：
10.1073/pnas.87.12.4781
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发表时间：
1990-06-01
影响因子：
11.1
通讯作者：
RAJEWSKY, K
中科院分区：
文献类型：
--
作者：
FORSTER, I;RAJEWSKY, K
我们已经重新评估了体内寿命的B淋巴细胞的基础上掺入的胸腺嘧啶核苷类似物5-溴-2 '-脱氧尿苷到DNA的分裂细胞。为了排除由于溴脱氧尿苷掺入而导致的B系细胞营业额的潜在改变，我们进行了脉冲追踪实验，比较了溴脱氧尿苷标记的细胞在长时间内的出现和消失。数据一致地显示，成年（大于2个月大）小鼠中超过三分之二的脾B细胞具有数周或数月的寿命，而在年轻（4周龄）小鼠中发生更快的更新。因此，外周血B细胞池在生命早期最初建立后才缓慢更新。
We have reevaluated the in vivo lifespan of B lymphocytes based on incorporation of the thymidine analogue 5-bromo-2'-deoxyuridine into the DNA of dividing cells. To exclude potential alterations in the turnover of B-lineage cells due to the bromodeoxyuridine incorporation, we performed pulse-chase experiments comparing the appearance and disappearance of bromodeoxyuridine-labeled cells over long periods of time. The data consistently show that more than two-thirds of splenic B cells in adult (greater than 2 months old) mice have a lifetime of several weeks or months, whereas a more rapid turnover takes place in young (4 week old) mice. Thus, the peripheral B-cell pool is only slowly renewed after it has been initially built up early in life.