Protein expression of fatty acid transporter 2 is polarized to the trophoblast basal plasma membrane and increased in placentas from overweight/obese women.
Protein expression of fatty acid transporter 2 is polarized to the trophoblast basal plasma membrane and increased in placentas from overweight/obese women.
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DOI：
10.1016/j.placenta.2016.02.010
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发表时间：
2016-04
期刊：
影响因子：
3.8
通讯作者：
Powell TL
中科院分区：
文献类型：
--
作者：
Lager S;Ramirez VI;Gaccioli F;Jang B;Jansson T;Powell TL
肥胖和超重的妇女更有可能生下一个大婴儿或一个肥胖增加的婴儿，但其潜在的机制还没有很好地建立起来。我们检验了超重/肥胖妇女胎盘转运脂肪酸的能力增加的假设。招募了57名体重指数（BMI）范围为18.4至54.3 kg/m2的无并发症足月妊娠孕妇，采集血样和胎盘组织。测量血浆中非酯化脂肪酸（NEFA）的母体和胎儿水平。CD 36/脂肪酸转位酶（FAT），脂肪酸转运蛋白（FATP）2和FATP 4的表达和定位在固定的胎盘组织和分离的合体滋养层质膜从正常和高BMI母亲。母亲和胎儿血浆NEFA水平不相关（n=42）。FATP 2和FATP 4在基底质膜（BPM）中的表达比在微绒毛膜中的每单位膜蛋白高约4倍（P<0.001; n=7）。BPM中FATP 2的表达与母亲BMI相关（P<0.01; n=30），而CD 36/FAT和FATP 4的表达与母亲BMI无关。FATP向BPM的极化将促进脂肪酸通过胎盘转移。在超重/肥胖妊娠中，FATP 2表达增加可能导致向胎儿输送的脂肪酸增加，虽然我们没有直接数据，但我们推测这可能导致胎儿生长加速或脂肪沉积增加。
Obese and overweight women are more likely to deliver a large infant or an infant with increased adiposity, however the underlying mechanisms are not well established. We tested the hypothesis that placental capacity to transport fatty acid is increased in overweight/obese women. Fifty-seven pregnant women with body mass index (BMI) ranging from 18.4 to 54.3 kg/m2 and with uncomplicated term pregnancies were recruited for collection of blood samples and placental tissue. Maternal and fetal levels of non-esterified fatty acids (NEFAs) were measured in plasma. The expression and localization of CD36/fatty acid translocase (FAT), fatty acid transport protein (FATP)2, and FATP4 was determined in fixed placental tissue and in isolated syncytiotrophoblast plasma membranes from normal and high BMI mothers. Maternal and fetal plasma NEFA levels did not correlate (n=42). FATP2 and FATP4 expressions were approximately four-fold higher in the basal plasma membrane (BPM) compared to the microvillous membrane (P<0.001; n=7) per unit membrane protein. BPM expression of FATP2 correlated with maternal BMI (P<0.01; n=30); there was no association between CD36/FAT or FATP4 expression and maternal BMI. The polarization of FATPs to the BPM will facilitate fatty acid transfer across the placenta. In overweight/obese pregnancies, the increased FATP2 expression could contribute to increased fatty acid delivery to the fetus and while we have no direct data we speculate that this could lead accelerated fetal growth or increased fat deposition.