Androgen and Luteinizing Hormone Stimulate the Function of Rat Immature Leydig Cells Through Different Transcription Signals.
Androgen and Luteinizing Hormone Stimulate the Function of Rat Immature Leydig Cells Through Different Transcription Signals.
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雄激素和黄体生成素通过不同转录信号刺激大鼠未成熟间质细胞的功能
DOI：
10.3389/fendo.2021.599149
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发表时间：
2021
影响因子：
5.2
通讯作者：
Ge RS
中科院分区：
文献类型：
--
作者：
Li X;Zhu Q;Wen Z;Yuan K;Su Z;Wang Y;Zhong Y;Ge RS
未成熟间质细胞的功能受激素如雄激素和促黄体生成素（LH）的调节。然而，这一过程的监管仍不清楚。本研究的目的是确定是否促黄体生成素（LH）或雄激素有助于这一过程。从35日龄雄性Sprague道利大鼠中纯化未成熟Leydig细胞，并用LH（1 ng/ml）或雄激素（7α-甲基-19-去甲睾酮，MENT，100 nM）培养2天。LH或MENT处理显著增加大鼠未成熟Leydig细胞产生的雄激素。基因芯片、qPCR和酶法检测结果显示，LH上调Scarb 1、Cyp 11 a1、Cyp 17 a1和Srd 5a 1的表达，下调Sult 2a 1和Akr 1c 14的表达。而CYP 17 a1的表达则被MENT上调。LH和MENT通过不同的转录因子调节Leydig细胞的功能。我们的结论是，LH和雄激素通过不同的转录途径参与调节大鼠未成熟Leydig细胞的功能。
The function of immature Leydig cells is regulated by hormones, such as androgen and luteinizing hormone (LH). However, the regulation of this process is still unclear. The objective of this study was to determine whether luteinizing hormone (LH) or androgens contribute to this process. Immature Leydig cells were purified from 35-day-old male Sprague Dawley rats and cultured with LH (1 ng/ml) or androgen (7α-methyl-19- nortestosterone, MENT, 100 nM) for 2 days. LH or MENT treatment significantly increased the androgens produced by immature Leydig cells in rats. Microarray and qPCR and enzymatic tests showed that LH up-regulated the expression of Scarb1, Cyp11a1, Cyp17a1, and Srd5a1 while down-regulated the expression of Sult2a1 and Akr1c14. On the contrary, the expression of Cyp17a1 was up-regulated by MENT. LH and MENT regulate Leydig cell function through different sets of transcription factors. We conclude that LH and androgens participate in the regulation of rat immature Leydig cell function through different transcriptional pathways.
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影响因子：
64.8
作者：
FOULKES, NS;MELLSTROM, B;SASSONECORSI, P
通讯作者：
SASSONECORSI, P
DOI：
10.1096/fj.14-255729
发表时间：
2015-03
期刊：
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
影响因子：
--
作者：
O'Hara L;McInnes K;Simitsidellis I;Morgan S;Atanassova N;Slowikowska-Hilczer J;Kula K;Szarras-Czapnik M;Milne L;Mitchell RT;Smith LB
通讯作者：
Smith LB
影响因子：
--
作者：
Lei, ZM;Mishra, S;Rao, CV
通讯作者：
Rao, CV
影响因子：
--
作者：
Jo, Misung;Curry, Thomas E., Jr.
通讯作者：
Curry, Thomas E., Jr.
影响因子：
4.8
作者：
HARDY, MP;ZIRKIN, BR;EWING, LL
通讯作者：
EWING, LL