Chemosensory proteins in the honey bee:: Insights from the annotated genome, comparative analyses and expressional profiling
Chemosensory proteins in the honey bee:: Insights from the annotated genome, comparative analyses and expressional profiling
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DOI：
10.1016/j.ibmb.2006.09.009
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发表时间：
2007-01-01
影响因子：
3.8
通讯作者：
Maleszka, Ryszard
中科院分区：
文献类型：
--
作者：
Foret, Sylvain;Wanner, Kevin W.;Maleszka, Ryszard
小化学感觉蛋白（CSPs）属于一个保守的，但知之甚少的蛋白质家族，涉及在昆虫的感受器内运输化学刺激。然而，它们的表达模式表明，这些分子对包括早期发育在内的其他功能也至关重要。本文采用生物信息学和实验相结合的方法对群居昆虫西方蜜蜂的CSP基因家族进行了表征，并将其成员与其他节肢动物的CSP基因家族进行了比较。蜜蜂基因组中的csp数量（6个）与双翅目物种（4 - 7个）相似，但远低于飞蛾或甲虫（各约20个）的csp数量。这些差异似乎是谱系特定扩展的结果。我们对许多节肢动物的CSPs分析揭示了一个保守的基因家族，在下颌骨和螯肢动物中都有发现。在不同组织和整个发育过程中的表达谱揭示了比预期更广泛的表达模式，没有一种csp局限于触角，一种csp仅在后卵巢和胚胎中发现。我们得出结论，csp是一种多功能的上下文依赖蛋白，参与从胚胎发育到化学感觉信号转导的多种细胞过程。一些csp可能在角质层合成中起作用，这与它们在节肢动物中的进化起源一致。(c) 2006 Elsevier Ltd.版权所有。
Small chemosensory proteins (CSPs) belong to a conserved, but poorly understood protein family that has been implicated in transporting chemical stimuli within insect sensilla. However, their expression patterns suggest that these molecules are also critical for other functions including early development. Here we used both bioinformatics and experimental approaches to characterize the CSP gene family in a social insect, the Western honey bee Apis mellifera, and then compared its members to CSPs in other arthropods. The number of CSPs in the honey bee genome (six) is similar to that found in the sequenced dipteran species (four-seven), but is much lower than the number of CSPs in the moth or in the beetle (around 20 each). These differences seem to be the result of lineage specific expansions. Our analysis of CSPs in a number of arthropods reveals a conserved gene family found in both Mandibulates and Chelicerates. Expressional profiling in diverse tissues and throughout development reveals broader than expected patterns of expression with none of the CSPs restricted to the antennae and one found only in the queen ovaries and in embryos. We conclude that CSPs are multifunctional context-dependent proteins involved in diverse cellular processes ranging from embryonic development to chemosensory signal transduction. Some CSPs may function in cuticle synthesis, consistent with their evolutionary origins in the arthropods. (c) 2006 Elsevier Ltd. All rights reserved.