1H MRS in the rat brain under pentobarbital anesthesia:: Accurate quantification of in vivo spectra in the presence of propylene glycol
1H MRS in the rat brain under pentobarbital anesthesia:: Accurate quantification of in vivo spectra in the presence of propylene glycol
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DOI：
10.1002/mrm.21502
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发表时间：
2008-03-01
影响因子：
3.3
通讯作者：
Henry, Pierre-Gilles
中科院分区：
文献类型：
--
作者：
Iltis, Isabelle;Marjanska, Malgorzata;Henry, Pierre-Gilles
戊巴比妥麻醉的商业溶液通常含有水、乙醇和丙二醇（PG）。后两者的特点是共振，可以影响代谢物浓度的测定从H-1光谱。本研究的目的是利用H-1 mrs测量戊巴比妥麻醉下大鼠脑内代谢物的浓度，在大鼠脑内观察PG的共振，而不是乙醇的共振。在37℃和pH 7.1条件下测量PG的化学位移和j -耦合常数，并用于光谱模拟。将模拟的PG光谱包含在LCModel分析的基集中，可以准确地拟合体内光谱。这项工作表明，在戊巴比妥麻醉下，使用H-1光谱可以可靠地测量脑代谢物的浓度。本文报道的化学位移和j -耦合值可用于模拟任意场强和不同脉冲序列下的PG光谱。
Commercial solutions for pentobarbital anesthesia typically contain water, ethanol, and propylene glycol (PG). The last two are characterized by resonances that can affect the determination of metabolite concentrations from H-1 spectra. The purpose of the present study was to measure the concentration of metabolites in the rat brain in vivo under pentobarbital anesthesia using H-1 MRS. Resonances of PG, but not ethanol, were observed in the rat brain. Chemical shifts and J-coupling constants for PG were measured at 37 degrees C and pH 7.1 and used for spectral simulation. Inclusion of the simulated PG spectrum in the basis set for LCModel analysis enabled accurate fitting of in vivo spectra. This work demonstrates that concentration of brain metabolites can be reliably measured using H-1 spectroscopy under pentobarbital anesthesia. The chemical shifts and J-coupling values reported here can be used to simulate the spectrum of PG at any field strength, with various pulse sequences.