Rat bone marrow stromal cells-seeded porous gelatin/tricalcium phosphate/oligomeric proanthocyanidins composite scaffold for bone repair
Rat bone marrow stromal cells-seeded porous gelatin/tricalcium phosphate/oligomeric proanthocyanidins composite scaffold for bone repair
复制标题
DOI：
10.1002/term.1461
复制
发表时间：
2013-09-01
影响因子：
3.3
通讯作者：
Yao, Chun-Hsu
中科院分区：
文献类型：
--
作者：
Chen, Kuo-Yu;Chung, Chia-Mei;Yao, Chun-Hsu
骨缺损的修复仍然是骨科手术的主要挑战。骨组织工程是治疗各种形状和数量的骨丢失的一种有吸引力的方法。本研究的目的是制备和评价多孔支架的可行性，该支架由低聚原花青素交联明胶与磷酸三钙（GTP）混合组成，并接种骨髓基质细胞（BMSCs）作为骨替代物。使用盐浸法使GTP支架多孔。评价支架的物理化学性质以确定最佳盐：复合物重量比。结果表明，当盐与复合物的重量比为4：1时，GTP支架具有有利的大孔结构和较高的孔隙率。细胞毒性试验表明，GTP支架提取物促进BMSCs增殖。将大鼠骨髓基质细胞接种在GTP支架上，并在转瓶中培养。培养2周后，扫描电镜观察显示细胞与支架孔隙表面粘附良好。此外，本研究还探讨了大鼠颅骨对该支架的生物学反应，以评价其在骨组织工程中的应用潜力。骨缺损分别用BMSC种植的GTP支架和脱细胞的GTP支架填充。8周后，支架诱导骨缺损处的新骨形成，如通过X射线显微放射照相和组织学所证实的。BMSC接种的支架比脱细胞支架诱导更多的新骨形成。这些观察结果表明，BMSC接种的GTP支架可以促进有缺陷的骨组织的再生。版权所有（c）2012约翰威利父子有限公司
Repair of bone defects remains a major challenge in orthopaedic surgery. Bone tissue engineering is an attractive approach for treating bone loss in various shapes and amounts. The aim of this study was to prepare and evaluate the feasibility of a porous scaffold, which was composed of oligomeric proanthocyanidin crosslinked gelatin mixed with -tricalcium phosphate (GTP) and was seeded with bone marrow stromal cells (BMSCs) as a bone substitute. GTP scaffolds were made porous using a salt-leaching method. The physicochemical properties of the scaffold were evaluated to determine the optimal salt:composite weight ratio. The results indicated that the GTP scaffold had a favourable macroporous structure and higher porosity when the salt:composite weight ratio was 4:1. Cytotoxic tests demonstrated that extracts from the GTP scaffolds promoted the proliferation of BMSCs. Rat BMSCs were seeded on a GTP scaffold and cultured in a spinner flask. After 2weeks of culture, scanning electron microscopy observation showed that the cells adhered well to the surfaces of the pores in the scaffold. Moreover, this study explored the biological response of rat calvarial bone to the scaffold to evaluate its potential in bone tissue engineering. Bone defects were filled with BMSC-seeded GTP scaffold and acellular GTP scaffold. After 8weeks, the scaffold induced new bone formation at a bone defect, as was confirmed by X-ray microradiography and histology. The BMSC-seeded scaffold induced more new bone formation than did an acellular scaffold. These observations suggest that the BMSCs-seeded GTP scaffold can promote the regeneration of defective bone tissue. Copyright (c) 2012 John Wiley & Sons, Ltd.