N-acetylcysteine alleviates liver injury by suppressing macrophage-mediated inflammatory response post microwave ablation
N-acetylcysteine alleviates liver injury by suppressing macrophage-mediated inflammatory response post microwave ablation
复制标题
N-乙酰半胱氨酸通过抑制微波消融后巨噬细胞介导的炎症反应减轻肝损伤
DOI：
10.1016/j.intimp.2020.106580
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发表时间：
2020-08-01
影响因子：
5.6
通讯作者：
Kong, Lianbao
中科院分区：
文献类型：
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作者：
Zhou, Haoming;Sun, Yu;Kong, Lianbao
目的：探讨n -乙酰半胱氨酸（NAC）对微波消融（MWA）大鼠肝损伤及全身炎症反应的缓解作用。材料与方法：体重150 ~ 200 g的雄性Sprague-Dawley大鼠随机分为假手术组（除MWA外仅麻醉开腹，根据不同情况分别腹腔注射PBS或NAC溶液）、对照组（MWA前2 h腹腔注射PBS进行比较）和NAC治疗组（MWA前2 h腹腔注射n -乙酰半胱氨酸（300 mg/kg））。根据肝损伤程度曲线，于术后4 h处死实验大鼠。采集肝组织和血清标本，检测病理、细胞凋亡、巨噬细胞含量和蛋白表达。结果：MVA处理大鼠血清肝酶、髓过氧化物酶（MPO）和炎症因子（tnf - α和CXCL1）水平升高显示MVA的损伤和促炎作用。MWA暴露大鼠的巨噬细胞聚集现象类似。NAC预处理可减轻肝损伤和肝细胞凋亡，以及肝组织中巨噬细胞的积累和随后的炎症反应。结论：应用n -乙酰半胱氨酸可减轻微波消融肝中巨噬细胞聚集和炎症反应，减轻肝损伤和细胞凋亡。
Object: To investigate N-acetyl-cysteine (NAC) would able to alleviate liver injury and systemic inflammatory response caused by microwave ablation (MWA) in rats.Materials and methods: Male Sprague-Dawley rats weighing 150-200 g were randomly divided into sham group (only anesthesia and laparotomy except MWA but with intraperitoneal PBS or NAC solution injection according to different situations), control group (intraperitoneal PBS injection for comparation 2 h prior to MWA), and NAC-treated group (intraperitoneal N-acetyl-cysteine (300 mg/kg) injection 2 h prior to MWA). Experimental rats were sacrificed at 4 h following operation in line with the liver injury severity curve. Liver tissue and serum samples were collected for determination of pathology, apoptosis, macrophages contents and protein expression.Results: The elevated serum level of liver enzymes, Myeloperoxidase (MPO) and inflammatory factors (TNF-alpha and CXCL1) in MWA-treated rats revealed injurious and pro-inflammatory effect of MVA. Macrophages aggregation was detected in MWA exposure rats similarly. and NAC pre-conditioning mitigate liver damage and hepatocyte apoptosis, besides macrophages accumulation and following inflammatory response in liver tissue.Conclusion: Our results demonstrated that N-acetyl-cysteine application alleviate macrophages aggregation and inflammatory response in liver suffering microwave ablation, and mitigating liver injury and cell apoptosis.