Role of spinal opioid receptor on the antiallodynic effect of intrathecal nociceptin in neuropathic rat
Role of spinal opioid receptor on the antiallodynic effect of intrathecal nociceptin in neuropathic rat
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DOI：
10.1016/j.neulet.2013.03.026
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发表时间：
2013-05-10
影响因子：
2.5
通讯作者：
Yoon, Myung Ha
中科院分区：
文献类型：
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作者：
Ju, Jin;Shin, Dong Jin;Yoon, Myung Ha
本研究的目的是检查鞘内痛觉肽对神经性疼痛的作用，并确定脊髓阿片受体类型的作用。用结扎L5、L6脊神经的方法诱导雄性sd大鼠神经性疼痛。几种拮抗剂鞘内注射，以评估伤害肽的作用机制：非选择性阿片受体拮抗剂（纳洛酮），mu阿片受体拮抗剂（CTOP）， delta阿片受体拮抗剂（纳曲多）和kappa阿片受体拮抗剂（GNTI）。Western blotting检测大鼠阿片受体蛋白水平。鞘内镇痛肽产生剂量依赖性抗疼痛。鞘内纳洛酮逆转了痛觉肽的抗痛觉作用。鞘内注射CTOP、纳曲多和GNTI逆转了痛觉肽的抗痛觉作用。Western blots显示脊髓阿片受体蛋白水平在神经性疼痛大鼠和正常大鼠之间没有差异。鞘内伤害肽使8类阿片受体蛋白水平较结扎大鼠升高，而mu、kappa类阿片受体蛋白水平不变。提示鞘内镇痛素对脊神经结扎引起的神经性疼痛具有抗异动作用。所有三种类型的脊髓mu， delta和kappa阿片受体都参与了伤害肽的抗异动机制。2013爱思唯尔爱尔兰有限公司版权所有。
The purpose of this study was to examine the effects of intrathecal nociceptin for neuropathic pain and determine the role of spinal opioid receptor types. Neuropathic pain was induced by ligation of L5 and L6 spinal nerves in male Sprague-Dawley rats. Several antagonists were intrathecally administered to evaluate the action mechanisms of nociceptin: nonselective opioid receptor antagonist (naloxone), mu opioid receptor antagonist (CTOP), delta opioid receptor antagonist (naltrindole) and kappa opioid receptor antagonist (GNTI). The levels of opioid receptor proteins were examined by Western blotting. Intrathecal nociceptin produced dose-dependent antiallodynia. Intrathecal naloxone reversed the antinociception of nociceptin. Intrathecal CTOP, naltrindole and GNTI reversed the antinociceptive effect of nociceptin. Western blots showed that the levels of spinal opioid receptor proteins did not differ between rats with neuropathic pain and nave rats. Intrathecal nociceptin increased the level of 8 opioid receptor protein compared with that of nerve ligated rats, while the levels of mu, and kappa opioid receptor proteins were unchanged. These results suggest that intrathecal nociceptin produced antiallodynic effect in spinal nerve ligation-induced neuropathic pain. All three types of spinal mu, delta, and kappa opioid receptors were involved in the antiallodynic mechanism of nociceptin. (C) 2013 Elsevier Ireland Ltd. All rights reserved.