A Rapid, Sensitive Thin Layer Chromatography Procedure for the Detection of Fumonisin B1 and B2
A Rapid, Sensitive Thin Layer Chromatography Procedure for the Detection of Fumonisin B1 and B2
复制标题
DOI：
10.1177/104063879200400316
复制
发表时间：
1992-07
影响因子：
1.5
通讯作者：
G. Rottinghaus;Charles E. Coatney;H. Minor
中科院分区：
文献类型：
--
作者：
G. Rottinghaus;Charles E. Coatney;H. Minor
开发了薄层色谱 (TLC) 方法用于检测玉米和玉米饲料中的伏马菌素 B1 和 B2。精细研磨的样品用乙腈:水 (1:1) 萃取、过滤并上样至 C18 净化柱。用1% KCl水溶液洗涤柱，然后用乙腈:1% KCl水溶液(1:9)洗涤，并用乙腈:水(7:3)洗脱伏马菌素。将洗脱液浓缩并与伏马菌素 B1 和 B2 标准品一起点样在反相 C18 TLC 板上，并将板在甲醇：4% KCl 水溶液 (3:2) 中展开。通过用 0.1 M 硼酸钠缓冲液、荧光胺和 0.01 M 硼酸连续喷雾 TLC 板来观察伏马菌素。然后将板干燥并在长波紫外光下检查。伏马菌素 B1 和 B2 在 Rf 分别为 0.5 和 0.1 时呈现明亮的黄绿色荧光带。 TLC 板上伏马菌素在玉米中的检测限为 0.1 ppm。加标样品的回收率平均>80%。通过将 B1 和 B2 的母体化合物水解为其各自的 C22 氨基醇并使用相同的可视化试剂通过 TLC 重新检查来确认伏马菌素的鉴定。该程序用于调查 1990 年从密苏里大学试验田收集的 193 个玉米样品中的伏马菌素 B。 15% 的玉米样品中检测到伏马菌素 B1。
A thin layer chromatography (TLC) method was developed for the detection of fumonisin B1 and B2 in corn and corn-based feedstuffs. Finely ground samples were extracted with acetonitrile: water (1: 1), filtered, and applied to C18 cleanup columns. The columns were washed with 1% aqueous KCl followed by acetonitrile: 1% aqueous KC1 (1:9), and the fumonisins were eluted with acetonitrile: water (7:3). The eluants were concentrated and spotted on reverse-phase C18 TLC plates along with fumonisin B1 and B2 standards, and the plates were developed in methanol: 4% aqueous KCl (3:2). The fumonisins were visualized by spraying the TLC plates successively with 0.1 M sodium borate buffer, fluorescamine, and 0.01 M boric acid. The plates were then dried and examined under longwave ultraviolet light. Fumonisin B1 and B2 appeared as bright yellowish-green fluorescent bands at Rfs of 0.5 and 0.1, respectively. The detection limit for the fumonisins on the TLC plate was 0.1 ppm in corn. Recoveries from spiked samples averaged >80%. The identification of the fumonisins was confirmed by hydrolyzing the parent compounds of B1 and B2 to their respective C22 amino-alcohols and reexamining by TLC with the same visualizing reagents. This procedure was used to survey 193 corn samples collected from University of Missouri test plots in 1990 for fumonisin B,. Fumonisin B1 was detected in 15% of the corn samples.