Rapid aggregation of gold nanoparticles induced by non-cross-linking DNA hybridization
Rapid aggregation of gold nanoparticles induced by non-cross-linking DNA hybridization
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DOI：
10.1021/ja034876s
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发表时间：
2003-07-09
影响因子：
15
通讯作者：
Maeda, M
中科院分区：
文献类型：
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作者：
Sato, K;Hosokawa, K;Maeda, M
迄今为止，通过杂交靶DNA在交联配置的DNA功能化金纳米粒子聚集已被深入研究。在这里，我们报告了非交联结构的聚合也是可能的，并且由于其速度和灵敏度，从遗传分析的角度来看，它甚至更好。在该系统中，用（烷硫醇）-15mer DNA功能化的直径为15nm的金纳米颗粒在室温下与15mer DNA杂交。在高NaCl浓度（≥0.5 M）下，与互补的靶DNA杂交可通过盐析效应诱导纳米颗粒聚集。这种聚集可以在3分钟内通过胶体溶液的比色变化来检测。此外，还发现该系统对锚定侧对面端单碱基失配的异常敏感性。事实上，具有这种错配的靶DNA根本不会引起比色变化，而中间有单碱基错配的靶DNA则无法与完全互补的靶DNA区分开来。这种非交联聚合系统为快速可靠的遗传分析开辟了新的可能性。
To date, aggregation of DNA-functionalized gold nanoparticles by hybridization of target DNA in a cross-linking configuration has been intensively studied. Here, we report that aggregation in a non-cross-linking configuration is also possible and is even better from the viewpoint of genetic analysis because of its speed and sensitivity. In this system, 15 nm diameter gold nanoparticles functionalized with (alkanethiol)-15mer DNA are hybridized to target 15mer DNA at room temperature. At high NaCl concentration (≥0.5 M), hybridization with complementary target DNA induces nanoparticle aggregation based on the salting-out effect. The aggregation can be detected by a colorimetric change of the colloidal solution within 3 min. Furthermore, unusual sensitivity of this system for single-base mismatch at the terminus opposite to the anchored side has been discovered. In fact, target DNA with such a kind of mismatch does not induce the colorimetric change at all, while target DNA with single-base mismatch at the middle of it cannot be discriminated from the fully complementary target. This non-cross-linking aggregation system opens up a new possibility of rapid and reliable genetic analysis.