Continuous acetone-butanol production with free and immobilized Clostridium acetobutylicum
Continuous acetone-butanol production with free and immobilized Clostridium acetobutylicum
复制标题
利用游离和固定化丙酮丁醇梭菌连续生产丙酮-丁醇
DOI：
10.1007/bf00253646
复制
发表时间：
1986
影响因子：
5
通讯作者：
K. Schügerl
中科院分区：
文献类型：
--
作者：
Ch. Frick;K. Schügerl
丙酮-丁醇的生产是用丙酮丁醇梭菌DSM 792（ATCC 824）在两级搅拌罐级联中进行的，培养基体积为1.25升和2.5升，使用游离细胞和固定化细胞。用海藻酸钠、κ-卡拉胶和/或壳聚糖固定化细胞。将含有细胞的小球干燥或化学处理以改善其长期稳定性。干燥的海藻酸钙产生最好的基质系统。它在搅拌釜反应器中发酵727 h后保持稳定。在15.4 g l-1的溶剂浓度下，游离细胞的溶剂（丙酮、丁醇和乙醇的总和）生产率为1.93 g l-1h-1，在4.0 g l-1h-1的溶剂浓度下，海藻酸钙固定化细胞（25%细胞负载，12 g l-1沉淀浓度，3 g l-1湿细胞质量浓度）的溶剂生产率增加到4.02 g l-1h-1。当颗粒直径为0.5mm时，生物催化剂效率低于50%。
Acetone-butanol production was carried out withClostridium acetobutylicumDSM 792 (ATCC 824) in a two-stage stirred tank cascade with 1.25 l and 2.5 l medium volume using free and immobilized cells. The cells were immobilized by alginate, κ-carrageenan and/or chitosan. The cell-containing pellets were dried or chemically treated to improve their long-term stability. Dried Ca-alginate yielded the best matrix system. It remained stable after a fermentation time of 727 h in stirred tank reactors. The solvent (sum of acetone, butanol and ethanol) productivity of 1.93 g l-1h-1at a solvent concentration of 15.4 g l-1with free cells was increased to 4.02 g l-1h-1at a solvent concentration of 4.0 g l-1h-1with Ca-alginate immobilized cells (25% cell loading, 12 g l-1pellet concentration, 3 g l-1wet cell mass concentration). With 0.5 mm pellet diameter, the biocatalyst efficiency was lower than 50%.