Matrix effects in secondary ion mass spectrometric analysis of biological tissue.
Matrix effects in secondary ion mass spectrometric analysis of biological tissue.
复制标题
生物组织二次离子质谱分析中的基质效应。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
P. Gallé
中科院分区：
文献类型：
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作者：
M. Burns;D. File;V. Deline;P. Gallé
我们在研究过程中进行了几次观察，结果表明软生物组织和标准品中存在基质效应。明胶的溅射速率约为环氧树脂的两倍，但明胶中的锂离子产额比环氧树脂小一个数量级。锇浸渍的冻干材料显着改变本地化的钙，但不是钾和钡。渗透组织中钙的绝对计数率比冻干组织中的钙的绝对计数率增加数倍。溅射材料的扫描电子显微镜显示在溅射期间锥的形成，其特别地但不排他地与黑色素颗粒和红细胞相关。已知这些结构对Na、K和Ca具有高发射性。植入硼的组织也表现出从黑色素颗粒选择性地发射硼。单原子和多原子发射的相对比例在环氧树脂、明胶和组织中不同。用掺钒环氧树脂包埋的组织中的碳、氯和钒的离子图像显示对应于组织结构的局部区域的变化。常见的二次离子的能量分布略有不同，在树脂和两个不同的组织区域。这些例子表明，在软生物组织中存在潜在的基质效应，涉及差分溅射和离子产额效应。
We have made several observations during the course of our studies that show the presence of matrix effects in soft biological tissue and standards. The sputtering rate of gelatin is approximately twice that of epoxy resin, but the ion yield of lithium in gelatin is an order of magnitude less than in epoxy. Osmium impregnation of freeze-dried material significantly alters the localization of calcium, but not potassium and barium. The absolute count rate for calcium in osmicated tissue is increased several-fold above that in freeze-dried tissue. Scanning electron microscopy of sputtered material shows the formation of cones during sputtering, which is particularly, but not exclusively, associated with melanin granules and red blood cells. These structures are known to be highly emissive for Na, K, and Ca. Boron implanted tissue also exhibits selective boron emission from melanin granules. Relative proportions of monoatomic and polyatomic emission vary in epoxy, gelatin and tissue. Ion images of carbon, chlorine and vanadium in tissue embedded with a vanadium-doped epoxy resin show variations in local regions that correspond to tissue structure. The energy distributions of common secondary ions differed somewhat in resin and two different tissue regions. These examples show the existence of potential matrix effects in soft biological tissue that involve both differential sputtering and ion yield effects.