Circulating CCL20: A potential biomarker for active vitiligo together with the number of Th1/17 cells
Circulating CCL20: A potential biomarker for active vitiligo together with the number of Th1/17 cells
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循环 CCL20：活动性白癜风的潜在生物标志物以及 Th1/17 细胞的数量。
DOI：
10.1016/j.jdermsci.2018.12.005
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发表时间：
2019-02-01
影响因子：
4.6
通讯作者：
Xiang, Leihong
中科院分区：
文献类型：
--
作者：
Zhang, Li;Kang, Yuli;Xiang, Leihong
背景：白癜风是一种自身免疫性疾病，具有不同的病理特征。CCL 20-CCR 6轴的激活在慢性炎性疾病中起重要作用。目的：探讨CCL 20和Th 1/17、Tc 1/17细胞在白癜风发病机制中的作用。使用酶联免疫吸附试验研究了血清和疱液IL-17、IFN-γ、CCL 20和CXCLI 0。使用流式细胞术定量循环中Th 1/17细胞和Tc 1/17细胞的数量。采用实时荧光定量聚合酶链反应（real-time polymerase chain reaction，PCR）检测外周血单个核细胞（peripheral blood mononuclear cells，PBMCs）中CCR 6 mRNA的表达水平，并采用蛋白质印迹法（western blotting）检测其蛋白水平。结果：白癜风患者血清中CCL 20水平明显升高。血清CCL 20水平在活动期高于稳定期，与白癜风欧洲工作组扩散评分和白癜风面积评分指数评分呈正相关。活动期白癜风患者外周血中Th 1/17细胞和Tc 1/17细胞数量增加，外周血单个核细胞和皮损中CCR 6表达上调。结论：CCL 20可能是活动期白癜风的重要生物标志物，循环中Th 1/17和Tc 1/17细胞参与了白癜风的发病过程。(C)2019日本皮肤病研究学会。Elsevier B. V.出版，保留所有权利。
Background: Vitiligo is an autoimmune disease with varying pathological features. Activation of the CCL20-CCR6 axis plays an important role in chronic inflammatory diseases. However, whether CCL20-CCR6 and Th1/17 cells are indicative of active vitiligo is unclear.Objective: To investigate the potential role of CCL20 and the involvement of Th1/17 and Tc1/17 cells in the mechanism in vitiligo.Methods: One hundred patients with vitiligo, and 20 healthy controls were included. The serum and blister fluid IL-17, IFN-gamma, CCL20, and CXCLI0 were studied using enzyme-linked immunosorbent assays. The numbers of Th1/17 cells and Tc1/17 cells in circulation were quantified using flow cytometry. CCR6 mRNA in peripheral blood mononuclear cells (PBMCs) was analyzed by real-time polymerase chain reaction and the protein level was confirmed by western blotting. CCR6 and CCL20 expression in lesions was analyzed by immunohistochemistry.Results: The serum CCL20 level was significantly elevated in patients with vitiligo. The level of serum CCL20 was higher in active than in the stable stage, which correlated positively with the Vitiligo European Task Force spreading score and the Vitiligo Area Scoring Index score. Patients with active vitiligo had elevated numbers of circulating Th1/17 cells and Tc1/17 cells, and upregulated expression of CCR6 in PBMCs and lesions. After effective treatment, the level of CCL20 in sera and blister fluid was significantly decreased, as were the numbers of circulating Th1/17 cells and Tc1/17 cells.Conclusion: CCL20 might be a vital biomarker of active vitiligo, and circulating Th1/17 and Tc1/17 cells are involved in the pathogenesis of vitiligo. (C) 2019 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.