Hollow fiber membrane-coated functionalized polymeric ionic liquid capsules for direct analysis of estrogens in milk samples
Hollow fiber membrane-coated functionalized polymeric ionic liquid capsules for direct analysis of estrogens in milk samples
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用于直接分析牛奶样品中雌激素的中空纤维膜涂层功能化聚合物离子液体胶囊
DOI：
10.1007/s00216-015-9279-9
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发表时间：
2016-02-01
影响因子：
4.3
通讯作者：
Luo, Chuannan
中科院分区：
文献类型：
--
作者：
Feng, Juanjuan;Sun, Min;Luo, Chuannan
对于牛奶样品的分析，蛋白质的去除过程总是很耗时的。本工作合成了中空纤维膜包裹功能化聚合物离子液体(HF-PIL)胶囊，并将其作为固相微萃取(SPME)吸附剂用于牛奶样品中雌激素的直接分析。通过1-(3-aminopropyl)-3-(4-vinylbenzyl)imidazolium-4-苯磺酸盐IL单体与1，6-二(3-乙烯基咪唑)正己烷双氟磷酸盐IL-交联剂的共聚反应，得到了功能化的PIL整体吸附剂。采用四种胶囊作为固相微萃取装置，用高效液相色谱法测定牛奶样品中的4种雌激素(雌酮、己烯雌酚、己雌酚和17-α-乙炔雌二醇)。对萃取和解吸条件进行了优化，获得了满意的萃取效果。L−1在5~2 0 0μg范围内线性关系良好，己烯雌酚的检出限为L−1，己烯雌酚为1μg，17−-乙炔雌二醇、雌酮和己雌酚的检出限为2αg。应用本方法对不同牛奶样品中的模型分析物进行了分析。相对回收率在85.5-112%之间。中空纤维膜包覆功能化聚合物离子液体固相微胶囊直接测定牛奶中的雌激素。
Protein removal process is always time-consuming for the analysis of milk samples. In this work, hollow fiber membrane-coated functionalized polymeric ionic liquid (HF-PIL) capsules were synthesized and used as solid-phase microextraction (SPME) sorbent for direct analysis of estrogens in milk samples. The functionalized PIL monolith sorbent was obtained by copolymerization between 1-(3-aminopropyl)-3-(4-vinylbenzyl)imidazolium 4-styrenesulfonate IL monomer and 1,6-di(3-vinylimidazolium) hexane bishexafluorophosphate IL-crosslinking agent. A group of four capsules were installed as SPME device, to determine four kinds of estrogens (estrone, diethylstilbestrol, hexestrol, and 17α-ethynylestradiol) in milk samples, coupled to high performance liquid chromatography. Extraction and desorption conditions were optimized to get satisfactory extraction efficiency. Good linearity was obtained in the range of 5–200 μg L−1. The limits of detection were 1 μg L−1for diethylstilbestrol and 2 μg L−1for 17α-ethynylestradiol, estrone, and hexestrol. The present method was applied to analyze the model analytes in different milk samples. Relative recoveries were in the range of 85.5–112 %. The HF-PIL SPME capsules showed satisfactory extraction efficiency and high resistance to sample matrix interference.Graphical AbstractDirect analysis of estrogens in milk samples by hollow fiber membrane-coated functionalized polymeric ionic liquids SPME capsules.