Typing of intimin (eae) enteropathogenic from children with genes from Escherichia coli (EPEC) isolated diarrhoea in Montevideo, Uruguay:: identification of two novel intimin variants (μB and ξR/β2B)
Typing of intimin (eae) enteropathogenic from children with genes from Escherichia coli (EPEC) isolated diarrhoea in Montevideo, Uruguay:: identification of two novel intimin variants (μB and ξR/β2B)
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DOI：
10.1099/jmm.0.46518-0
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发表时间：
2006-09-01
影响因子：
3
通讯作者：
Blanco, Jorge
中科院分区：
文献类型：
--
作者：
Blanco, Miguel;Blanco, Jesus E.;Blanco, Jorge
本研究对从乌拉圭蒙得维的亚腹泻儿童中分离的71株肠致病性大肠杆菌（EPEC）进行了鉴定。PCR结果显示，57株EPEC菌株同时携带eae和bfp基因（典型EPEC菌株），14株仅携带eae基因（非典型EPEC菌株）。这些EPEC菌株属于21个O：H血清型，包括8个以前在其他研究中未报告的人EPEC的新血清型。然而，72%的菌株仅属于4种血清型：O 55：H-（6株），O 111：H2（13株），O 111：H-（14株）和O 119：H6（18株）。在71株EPEC中检测到9种类型的粘附素，即α 1型（2株O 142）、β 1型（29株，其中O 111：H2 13株，O 111：H-14株）、γ 1型（3株O 55：H-）、θ型（5株，其中3株含H40抗原）、κ型（2株）、β 1型（1株）、λ型（1株）、mu B型（6株血清型O 55：H51和O 55：H-）和xi R/β 2 B型（22株，其中O 119：H6 18株）。作者在血清型为O 55：H51/H-和O 119：H6/H-的典型EPEC菌株中鉴定了两种新的紧密素基因（mu B和xi R/β 2 B）。测定了新的mu B和xi R/β 2变体基因的完整核苷酸序列。对44株代表性EPEC菌株进行XbaI酶切后PFGE分型。基因组DNA指纹图谱显示44种不同的限制性酶切图谱，菌株聚在12个组中。只有15个菌株聚集在6组密切相关（相似性> 85%）的PFGE图谱中，表明蒙得维的亚腹泻儿童中分离的EPEC菌株具有普遍的克隆多样性。
A total of 71 enteropathogenic Escherichia coli(EPEC) strains isolated from children with diarrhoea in Montevideo, Uruguay, were characterized in this study. PCR showed that 57 isolates carried eae and bfp genes (typical EPEC strains), and 14 possessed only the eae gene (atypical EPEC strains). These EPEC strains belonged to 21 O:H serotypes, including eight novel serotypes not previously reported among human EPEC in other studies. However, 72% belonged to only four serotypes: O55:H- (six strains), O111:H2 (13 strains), O111:H- (14 strains) and O119:H6 (18 strains). Nine intimin types, namely, alpha 1 (two O142 strains), beta 1 (29 strains, including 13 O111:H2 and 14 O111:H-), gamma 1 (three O55:H- strains), theta(five strains, including three strains with H40 antigen), kappa (two strains), epsilon 1 (one strain), lambda (one strain), mu B (six strains of serotypes O55:H51 and O55:H-) and xi R/beta 2B (22 strains, including 18 O119:H6) were detected among the 71 EPEC strains. The authors have identified two novel intimin genes (mu B and xi R/beta 2B) in typical EPEC strains of serotypes O55:H51/H- and O119:H6/H-. The complete nucleotide sequences of the novel mu B and xi R/beta 2 variant genes were determined. PFGE typing after Xbal DNA digestion was performed on 44 representative EPEC strains. Genomic DNA fingerprinting revealed 44 distinct restriction patterns and the strains were clustered in 12 groups. Only 15 strains clustered in six groups of closely related (similarity > 85%) PFGE patterns, suggesting the prevailing clonal diversity among EPEC strains isolated from children with diarrhoea in Montevideo.