INTERACTIONS BETWEEN HUMAN-FIBROBLASTS AND HELA-CELLS INVITRO
INTERACTIONS BETWEEN HUMAN-FIBROBLASTS AND HELA-CELLS INVITRO
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DOI：
10.1111/j.1768-322x.1984.tb00250.x
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发表时间：
1984-01-01
影响因子：
2.7
通讯作者：
KOTTARIDIS, SD
中科院分区：
文献类型：
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作者：
DELINASSIOS, JG;KOTTARIDIS, SD
HeLa 细胞和源自人类肿瘤基质或正常组织的成纤维细胞之间的共培养证明了彼此的亲和力。混合培养物（比例 1:1、1:2、2:1）中的两种细胞类型均正常增殖，如共培养长达 48 小时的 3H-胸苷标记指数估计所示。当成纤维细胞与HeLa的比例低于1:10时，成纤维细胞在连续传代后最终被消除。 3H-核苷酸可以在异源细胞之间以任一方向转移。细胞的接触对于这种现象至关重要。将标记从 HeLa 转移到成纤维细胞需要更长的相互作用时间，并且明显低于从成纤维细胞转移到 HeLa 的时间。掺入任一细胞类型 DNA 中的 3H-胸苷不能从一个细胞转移到另一个细胞。该模型提供了一种在体外研究肿瘤性 X 正常（或肿瘤基质）细胞相互作用的方法。
Affinity toward each other was demonstrated in co-cultures between HeLa cells and fibroblasts originating from human tumor stromal or normal tissues. Both cell types in the mixed cultures (ratio 1:1, 1:2, 2:1) proliferated normally as shown by 3H-thymidine labeling index estimation for up to 48 h of coculture. At ratios of fibroblasts: HeLa lower than 1:10, fibroblasts were eventually eliminated after serial passaging. 3H-nucleotides could be transferred between heterologous cells in either direction. Contact of cells was essential for this phenomenon. Transfer of the label from HeLa to fibroblasts required a longer interaction time and was evidently lower than the transfer from fibroblasts to HeLa. 3H-thymidine incorporated into the DNA of either cell type could not be transferred from one cell to another. The model provides a means for studying neoplastic X normal (or tumor stromal) cell interactions in vitro.