EMBRYONIC STEM-CELLS EXPRESS NEURONAL PROPERTIES IN-VITRO
EMBRYONIC STEM-CELLS EXPRESS NEURONAL PROPERTIES IN-VITRO
复制标题
DOI：
10.1006/dbio.1995.1085
复制
发表时间：
1995-04-01
影响因子：
2.7
通讯作者：
GOTTLIEB, DI
中科院分区：
文献类型：
--
作者：
BAIN, G;KITCHENS, D;GOTTLIEB, DI
小鼠胚胎干（ES）细胞培养的聚集体和暴露于视黄酸诱导表达多种表型通常与神经元。很大比例的治疗聚集体产生丰富的神经炎生长。用胰蛋白酶解离诱导的聚集体，并将细胞作为单层铺板，得到其中相当大百分比的细胞具有神经元外观的培养物。这些神经元样细胞表达III类β-微管蛋白和神经丝M亚单位。诱导培养物表达神经相关基因的转录本，包括神经丝L亚基、谷氨酸受体亚基、转录因子Brn-3和GFAP。神经丝L和GAD（67）和GAD（65）转录物的水平在诱导后显著升高。生理学研究表明，神经元样细胞产生动作电位并表达TTX敏感性钠通道，以及电压门控性钾通道和钙通道。我们的结论是，一个复杂的系统的神经元基因表达可以激活培养的ES细胞。该系统将有利于研究调节神经元分化的一些机制。(C)出版社：Academic Press
Mouse embryonic stem (ES) cells cultured as aggregates and exposed to retinoic acid are induced to express multiple phenotypes normally associated with neurons. A large percentage of treated aggregates produce a rich neuritic outgrowth. Dissociating the induced aggregates with trypsin and plating the cells as a monolayer results in cultures in which a sizable percentage of the cells have a neuronal appearance. These neuron-like cells express class III beta-tubulin and the neurofilament M subunit. Induced cultures express transcripts for neural-associated genes including the neurofilament L subunit, glutamate receptor subunits, the transcription factor Brn-3, and GFAP. Levels of neurofilament L and GAD(67) and GAD(65) transcripts rise dramatically upon induction. Physiological studies show that the neuron-like cells generate action potentials and express TTX-sensitive sodium channels, as well as voltage-gated potassium channels and calcium channels. We conclude that a complex system of neuronal gene expression can be activated in cultured ES cells. This system should be favorable for investigating some of the mechanisms that regulate neuronal differentiation. (C) 1995 Academic Press, Inc.