ABOVEGROUND SCREENING FOR GENOTYPIC DIFFERENCES IN CUCUMBER ROOT-GROWTH IN THE GREENHOUSE AND FIELD
ABOVEGROUND SCREENING FOR GENOTYPIC DIFFERENCES IN CUCUMBER ROOT-GROWTH IN THE GREENHOUSE AND FIELD
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DOI：
10.21273/jashs.117.6.1006
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发表时间：
1992-11-01
影响因子：
1.9
通讯作者：
DUVALL, R
中科院分区：
文献类型：
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作者：
GRUMET, R;BARCZAK, M;DUVALL, R
采用地下除草剂条带技术，建立了一种简便的地上研究黄瓜根系生长的方法。那些根长得更深或更快的植物能更快地接触到除草剂，并更快地表现出除草剂伤害症状。温室盆栽试验表明，0.25或0.50公斤辛/公顷可以产生不同的症状；症状表现时间随带距土壤表面深度的增加而增加。洗根实验证实，根长与响应时间有关。在田间试验中，反应时间和症状的严重程度随除草剂浓度、深度和离种子行距离的不同而变化，从而提供根在土壤中的位置的指示。对100多个不同黄瓜基因型在温室和田间根系生长速率的差异进行了试验。症状表达时间在基因型间呈正态分布；方差分析（ANOVA）显示显著的基因型差异。该系统可用于培养或生理研究，或用于非破坏性的选择和育种目的。如果除草剂放得足够深，以防止对子叶的损害，植物就能开花结果。化学名称：6-氯-N， N'-二乙基-1,3,5-三嗪-2,4-二胺（辛嗪）。
A simple, aboveground method to study cucumber (Cucumis sativus L.) root growth was developed using a subsurface herbicide banding technique. Those plants with roots that grow deeper or faster reach the herbicide sooner and exhibit herbicide injury symptoms sooner. Greenhouse pot trials showed that 0.25 or 0.50 kg simazine/ha could be used to produce distinctive symptoms; time to symptom expression increased with the depth of the band from the soil surface. Root washing experiments verified that root length was associated with response time. In field trials, response time and severity of symptoms varied with herbicide concentration, depth, and distance from the seed row, thereby providing an indication of where the roots were in the soil. About 100 diverse cucumber genotypes were tested for differences in root growth rate in the greenhouse and in the field. Time to symptom expression was normally distributed among the genotypes; analysis of variance (ANOVA) indicated significant genotypic differences. This system can be used for cultural or physiological studies, or nondestructively for selection and breeding purposes. If the herbicide is placed sufficiently deep to prevent damage to the cotyledons, the plants are capable of flowering and producing fruit. Chemical name used: 6-chloro-N, N'-diethyl-1,3,5-triazine-2,4-diamine (simazine).