CELL CYCLE EVENTS IN HYDROCORTISONE REGULATION OF ALKALINE PHOSPHATASE IN HELA S3 CELLS
CELL CYCLE EVENTS IN HYDROCORTISONE REGULATION OF ALKALINE PHOSPHATASE IN HELA S3 CELLS
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DOI：
10.1083/jcb.40.2.297
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发表时间：
1969-01-01
影响因子：
7.8
通讯作者：
BER, R
中科院分区：
文献类型：
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作者：
GRIFFIN, MJ;BER, R
在添加氢化可的松的培养基中生长的HeLa S_3细胞的异步培养中碱性磷酸酶的增加的特征在于10 - 12小时的滞后期。目前利用同步细胞群的研究表明：（a）与氢化可的松孵育至少8 - 10小时是碱性磷酸酶最大诱导所必需的;（B）由氢化可的松产生的酶活性的增加仅在细胞周期的合成期开始;（c）碱性磷酸酶活性在正常对照细胞周期内没有明显变化。放射性氢化可的松迅速分布到HeLa细胞中，而不管它们在细胞周期中的位置，这表明诱导效应在细胞周期中不受选择性渗透性的支配。当细胞在不含氢化可的松的新鲜培养基中孵育时，氢化可的松-1，2-[3H]从细胞扩散回到培养基中，并且在这些条件下，碱性磷酸酶诱导是完全可逆的。
The increase in alkaline phosphatase in asynchronous cultures of HeLa S3cells grown in medium supplemented with hydrocortisone is characterized by a lag period of 10–12 hr. Present studies utilizing synchronous cell populations indicate: (a) a minimum of 8–10 hr of incubation with hydrocortisone is necessary for maximum induction of alkaline phosphatase; (b) the increase in enzyme activity produced by hydrocortisone is initiated exclusively in the synthetic phase of the cell cycle; (c) alkaline phosphatase activity does not vary appreciably over a normal control cell cycle. Radioactive hydrocortisone is rapidly distributed into HeLa cells irrespective of their position in the cell cycle, indicating that inductive effects are not governed by selective permeability during the cell cycle. Hydrocortisone-1,2-[3H] diffuses back from the cell into the medium when the cells are incubated in fresh medium containing no hydrocortisone, and the alkaline phosphatase induction, under these conditions, is completely reversible.