Expression of IAP family proteins in esophageal cancer
Expression of IAP family proteins in esophageal cancer
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DOI：
10.1016/j.yexmp.2004.01.001
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发表时间：
2004-06-01
影响因子：
3.6
通讯作者：
Koike, M
中科院分区：
文献类型：
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作者：
Nemoto, T;Kitagawa, M;Koike, M
凋亡抑制蛋白(IAP)家族的成员，包括Survivin，已被报道在许多肿瘤中表达。然而，它们在食道癌中的表达还没有完全阐明。本研究采用实时定量RT-PCR方法检测了食道癌及其癌旁正常组织中IAP家族蛋白的表达。Survivin在食管癌组织中的表达显著高于正常组织(P<；0.05)。其他IAP家族蛋白包括cIAP1、cIAP2、NAIP和XIAP在癌组织中的表达强于正常黏膜，但差异无统计学意义。在肿瘤的组织类型上，低分化鳞癌的表达水平显著高于高分化鳞癌(P<；0.05)。癌组织中凋亡细胞比例与Survivin表达强度呈负相关(P<；0.05)。免疫组织化学染色显示Survivin在食管癌组织中有胞浆和胞核表达，原位杂交分析显示Survivin在胞浆中有表达。结果提示，IAP家族蛋白尤其是Survivin的表达可能与食管癌的生物学特性有关，如细胞凋亡。(C)2004 Elsevier Inc.保留所有权利。
Members of the inhibitor of apoptosis protein (IAP) family, including survivin, have been reported to be expressed in many tumors. However, their expression in esophageal cancer has not been clarified completely. We investigated the expression of mRNA for IAP family proteins in samples from esophageal cancers and their adjacent normal mucosa tissues by real-time quantitative RT-PCR. The survivin expression in esophageal cancer was significantly higher than that in normal mucosa (P < 0.05). Other IAP family proteins including cIAP1, cIAP2, NAIP and XIAP tended to show stronger expression in cancer tissue than normal mucosa, although the differences were not significant. As to the histological type of tumor, poorly differentiated squamous cell carcinomas exhibited significantly higher level of expression than well-differentiated carcinomas (P < 0.05). The proportion of apoptotic cells of cancer tissue inversely correlated with the intensity of survivin expression (P < 0.05). Immumohistochemical staining demonstrated cytoplasmic as well as nuclear expression of survivin in esophageal cancer, and further, in situ hybridization analysis demonstrated cytoplasmic expression of mRNA for survivin. The results suggest that the expression of IAP family proteins, especially survivin, may be associated with the biological character of esophageal cancer, such as apoptosis. (C) 2004 Elsevier Inc. All rights reserved.