The imbalance between regulatory and memory B cells accompanied by an increased number of circulating T-follicular helper cells in MOG-antibody-associated demyelination
The imbalance between regulatory and memory B cells accompanied by an increased number of circulating T-follicular helper cells in MOG-antibody-associated demyelination
复制标题
MOG 抗体相关脱髓鞘中调节性 B 细胞和记忆 B 细胞之间的不平衡伴随着循环滤泡辅助性 T 细胞数量的增加
DOI：
10.1016/j.msard.2019.101397
复制
发表时间：
2019-11-01
影响因子：
4
通讯作者：
Quan, Chao
中科院分区：
文献类型：
--
作者：
Li, Xiaoyang;Wang, Liang;Quan, Chao
目的：目的：探讨髓鞘少突胶质细胞糖蛋白（MOG）抗体相关性脱髓鞘病（NMOSD）患者T、B淋巴细胞亚群比例的变化。不同淋巴细胞亚群的频率，包括CD 19（+）CD 27（+）记忆B细胞、CD 19（+）CD 24（hi）CD 38（hi）和CD 19（+）CD 5（+）CD 1d（hi）调节性B细胞、FN-γ表达B细胞、表达IL-10的B细胞和CD 4（+）CXCR 5（+）ICOS（+）T滤泡辅助细胞（T-FH）采用流式细胞仪检测各组间的细胞凋亡率。CD19（+）、CD24（hi）、CD38（hi）、与健康对照相比，CD 19（+）CD 5（+）CD 1d（hi）调节性B细胞以及表达IL-10的B细胞在MOG抗体相关脱髓鞘中显著降低，而MOG抗体阳性组中CD 19（+）CD 27（+）记忆B细胞的频率显著较高。与健康对照组相比，MOG抗体阳性组中T-FH的频率显著较高。MOG抗体阳性组和AQP 4抗体阳性组的上述淋巴细胞分布无显著性差异。结论：MOG抗体相关脱髓鞘急性期B细胞的免疫调节功能明显受损，而T-FH细胞明显增加。尽管具有不同的临床特征，但MOG抗体相关脱髓鞘在急性复发期与AQP 4抗体阳性NMOSD具有相似的淋巴细胞特征。
Objective: To explore the alteration of T and B lymphocyte subsets proportions in myelin oligodendrocyte glycoprotein (MOG)-antibody-associated demyelination.Methods: 19 MOG-antibody-positive, 25 AQP4-antibody-positive and 25 double-negative NMOSD patients in the acute phase of the diseases were included in the study, as well as 29 healthy controls. The frequencies of different lymphocyte subsets, including CD19 (+)CD27(+) memory B cells, CD19(+)CD24(hi)CD38(hi), and CD19(+)CD5(+)CD1d(hi) regulatory B cells, FN-gamma expressing B cells, IL-10 expressing B cells and CD4(+)CXCR5(+)ICOS(+) T-follicular helper cells (T-FH) were measured via flow cytometry and compared among the four groups.Results: The frequencies of CD19(+)CD24(hi)CD38(hi), CD19(+)CD5(+)CD1d(hi) regulatory B cells as well as the IL-10 expressing B cells were significantly lower in the MOG-antibody-associated demyelination compared to the healthy controls, whereas the frequencies of CD19(+)CD27(+) memory B cells were significantly higher in the MOG-antibody-positive group. The frequencies of T-FH were significantly higher in the MOG-antibody-positive group as compared to the healthy controls. No significant difference was detected in the above mentioned lymphocytic profile between the MOG-antibody-positive and the AQP4-antibody-positive groups.Conclusions: The immuno-regulatory functions of B cells were significantly impaired whereas T-FH cells were markedly increased in the acute phase of MOG-antibody-associated demyelination. Despite having distinct clinical features, MOG-antibody-associated demyelination shared a similar lymphocytic profile with AQP4-antibody-positive NMOSD in the acute relapse phase.