The distinct response of phenanthrene enriched bacterial consortia to different PAHs and their degradation potential: a mangrove sediment microcosm study
The distinct response of phenanthrene enriched bacterial consortia to different PAHs and their degradation potential: a mangrove sediment microcosm study
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DOI：
10.1016/j.jhazmat.2019.120863
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发表时间：
2019-12-15
影响因子：
13.6
通讯作者：
Dong, Junde
中科院分区：
文献类型：
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作者：
Ahmad, Manzoor;Yang, Qingsong;Dong, Junde
了解多环芳烃（PAHs）的微生物群落演替和重要的降解微生物类群的识别是设计适当的生物修复策略的关键。在本研究中，两种不同的菲富集细菌聚生体针对高分子量（芘、苯并（a）芘和苯并（a）荧蒽）进行处理，并通过使用高重复序列Illumine测序和qPCR分析在分类学变异方面研究了响应。总体而言，多环芳烃的种类显著影响了土壤细菌群落的组成和相对丰度，而苯并（a）芘和苯并（a）荧蒽处理的土壤细菌群落没有明显差异。新鞘氨醇菌属、假单胞菌属、黄杆菌属、分枝杆菌属、Hoefiae和Algoriphagus在所有处理组中均占优势，表明它们可能是降解多环芳烃的关键类群。由于在所有处理组中革兰氏阴性菌PAH环羟化双加氧酶基因的丰度高于革兰氏阳性菌，我们推测革兰氏阴性菌可能在PAH降解中贡献更大。沉积物中含有丰富的多环芳烃降解菌群，降解的多环芳烃既包括低分子量的多环芳烃，也包括高分子量的多环芳烃，这些发现为红树林生态系统中多环芳烃的微生物修复提供了新的视角。
Understanding the microbial community succession to polycyclic aromatic hydrocarbons (PAHs) and identification of important degrading microbial groups are crucial for the designing of appropriate bioremediation strategies. In the present study, two distinct phenanthrene enriched bacterial consortia were treated against high molecular weight (Pyrene, Benzo (a) pyrene and Benzo (a) fluoranthene) and the response was studied in term of taxonomic variations by using High Throughput Illumine sequencing and qPCR analysis. Overall, the type of PAHs significantly affected the composition and the relative abundance of bacterial communities while no obvious difference was detected between bacterial communities of benzo (a) pyrene and benzo (a) fluoranthene treatments. Genera, Novosphingobiwn, Pseudomonas, Flavobacterium, Mycobacterium, Hoefiae, and Algoriphagus dominated all PAHs treatment groups indicating that they could be the key PAHs degrading phylotypes. Due to the higher abundance of gram-negative PAH-ring hydroxylating dioxygenase gene than that of gram-positive bacteria in all treated groups, we speculated that gram-negative bacteria may contribute more in the PAH degradation. The studied sediments harbored rich PAHs degrading bacterial assemblages involved in both low and high molecular weight PAHs and these findings provided new insight into the perspective of microbial PAHs bioremediation in the mangrove ecosystem.