Purification and characterization of testicular transferrin secreted by rat Sertoli cells.
Purification and characterization of testicular transferrin secreted by rat Sertoli cells.
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大鼠支持细胞分泌的睾丸转铁蛋白的纯化和表征。
DOI：
10.1042/bj2180313
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Griswold,MD
中科院分区：
文献类型：
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作者：
Skinner,MK;Cosand,WL;Griswold,MD
支持细胞合成并分泌转铁蛋白样蛋白（睾丸转铁蛋白）[Skinner和格里斯沃尔德（1980）生物化学杂志255，1923-1925]。本研究的目的是纯化和鉴定睾丸转铁蛋白，并将其与血清转铁蛋白进行比较。睾丸转铁蛋白从培养的大鼠支持细胞的培养基中获得，而血清转铁蛋白从大鼠血清中获得。这两种蛋白质的纯化与使用苯基琼脂糖凝胶疏水层析和转铁蛋白免疫亲和层析。纯化的蛋白质具有相似的分子量（75 000 Da）和氨基酸组成。从睾丸和血清转铁蛋白的胰蛋白酶肽的模式被认为是基本相同的反相高压液相色谱分析时。两种转铁蛋白的碳水化合物组成通过几种比色测定和g.l.c.睾丸转铁蛋白，从细胞培养基中分离，有增加量的葡萄糖，半乳糖和葡糖胺。与细胞培养基一起孵育的血清转铁蛋白也具有大量的相关葡萄糖。结果表明，睾丸转铁蛋白和血清转铁蛋白的结构非常相似，可能是同一基因在两个不同组织中表达的产物，睾丸和肝脏。然而，与这两种蛋白质相关的碳水化合物的量是不同的。
Sertoli cells synthesize and secrete a transferrin-like protein (testicular transferrin) [Skinner & Griswold (1980) J. Biol. Chem. 255, 1923-1925]. The purpose of the present study was to purify and characterize testicular transferrin and to compare it with serum transferrin. Testicular transferrin was obtained from the medium of cultured rat Sertoli cells, whereas serum transferrin was obtained from rat serum. Both proteins were purified with the use of phenyl-Sepharose hydrophobic chromatography and transferrin immunoaffinity chromatography. The purified proteins were shown to have similar molecular masses (75 000 Da) and amino acid compositions. The pattern of tryptic peptides from testicular and serum transferrin were found to be essentially the same when analysed by reverse-phase high-pressure liquid chromatography. The carbohydrate composition of both transferrins was determined by several colorimetric assays and g.l.c. Testicular transferrin, isolated from cell culture medium, had increased amounts of glucose, galactose and glucosamine. Serum transferrin that was incubated with cell culture medium also had a large amount of associated glucose. The results show that testicular transferrin and serum transferrin are structurally very similar and are possibly products of the same gene expressed in two different tissues, the testis and liver. However, the amount of carbohydrate associated with these two proteins is different.