CELLULAR AND HUMORAL FACTORS GOVERNING CANINE MIXED LYMPHOCYTE CULTURES AFTER RENAL TRANSPLANTATION II. CELLULAR
CELLULAR AND HUMORAL FACTORS GOVERNING CANINE MIXED LYMPHOCYTE CULTURES AFTER RENAL TRANSPLANTATION II. CELLULAR
复制标题
肾移植后控制犬混合淋巴细胞培养的细胞和体液因素 II。
DOI：
10.1097/00007890-197207000-00007
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发表时间：
1971
期刊：
影响因子：
6.2
通讯作者：
M. Johnson
中科院分区：
文献类型：
--
作者：
B. Hattler;J. Miller;M. Johnson
混合淋巴细胞培养（MLC）的反应性已被跟踪在狗之前，期间和之后的异位移植肾移植排斥反应。在主动排斥期间，受体（R）外周血中细胞反应性丧失，这可归因于排斥肾中免疫反应性细胞的截留。在此期间，与从正常静脉和动脉血中获得的样本相比，从引流同种异体移植物的血液中取出的淋巴细胞的MLC反应性降低。从排斥器官回收的淋巴细胞显示出对刺激供体（D）淋巴细胞的加速MLC反应性，而来自外周血的淋巴细胞的反应性降低。使用中性（I）犬的刺激淋巴细胞也观察到与肾淋巴细胞的类似反应性。排斥反应后，当MLC反应性恢复时，免疫力增加的状态可以通过在培养物中孵育较短时间后反应性发作的加速来证明。
Mixed lymphocyte culture (MLC) reactivity has been followed in dogs before, during, and after rejection of heterotopically transplanted kidney allografts. During the time of active rejection, there was a loss of cellular reactivity in recipient (R) peripheral blood which could be ascribed to entrapment of immunoreactive cells in the rejecting kidneys. In this period, lymphocytes removed from blood draining the allograft had diminished MLC reactivity when compared to samples obtained from normal venous and arterial blood. Lymphocytes recovered from the rejecting organ demonstrated an accelerated MLC responsiveness to stimulating donor (D) lymphocytes at a time when lymphocytes from peripheral blood had diminished reactivity. Similar reactivity with kidney lymphocytes was also seen using stimulating lymphocytes of indifferent (I) dogs. Following rejection, when MLC responsiveness returned, a state of increased immunity could be demonstrated by an acceleration in the onset of reactivity after incubation in culture for shorter time periods.