Developmental Changes for the Hemolymph Metabolome of Silkworm (Bombyx mori L.).
Developmental Changes for the Hemolymph Metabolome of Silkworm (Bombyx mori L.).
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家蚕血淋巴代谢组的发育变化（Bombyx mori L.）
DOI：
10.1021/acs.jproteome.5b00159
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发表时间：
2015-05-01
影响因子：
4.4
通讯作者：
Tang H
中科院分区：
文献类型：
--
作者：
Zhou L;Li H;Hao F;Li N;Liu X;Wang G;Wang Y;Tang H
蚕（Bombyx mori）是一种鳞翅目全代谢模式生物。为了了解其发育生物化学，我们使用 1 H NMR 光谱法表征了从第三龄到预蛹阶段的幼虫血淋巴代谢组，同时使用 GC-FID/MS 表征了血淋巴脂肪酸组成。我们明确指定了 60 多种代谢物，其中酪氨酸-o-β-葡萄糖醛酸、中康酸、高肌肽和吡啶甲酸是首次从蚕血淋巴中报道。磷酰胆碱是所有发育阶段中最丰富的代谢物，第三次和第四次蜕皮之前的时期除外。我们还发现血淋巴代谢组具有明显的发育依赖性，涉及多种途径，包括蛋白质生物合成、糖酵解、TCA循环、胆碱氨基酸、脂肪酸、嘌呤和嘧啶的代谢。大多数血淋巴氨基酸在四龄和预蛹期摄食期间有两次升高。第五龄第8天之前的主要血糖是海藻糖，而旋转后的主要血糖是葡萄糖。 C16:0、C18:0 及其不饱和形式是血淋巴中的主要脂肪酸。血淋巴代谢组的发育变化与膳食营养摄入、细胞膜、色素、蛋白质和能量代谢的生物合成有关。这些发现提供了有关蚕发育过程中动态代谢变化的重要生物化学信息。
Silkworm (Bombyx mori) is a lepidopteran-holometabolic model organism. To understand its developmental biochemistry, we characterized the larval hemolymph metabonome from the third instar to prepupa stage using 1H NMR spectroscopy whilst hemolymph fatty acid composition using GC-FID/MS. We unambiguously assigned more than 60 metabolites, among which tyrosine-o-β-glucuronide, mesaconate, homocarnosine, and picolinate were reported for the first time from the silkworm hemolymph. Phosphorylcholine was the most abundant metabolite in all developmental stages with exception for the periods before the third and fourth molting. We also found obvious developmental dependence for the hemolymph metabonome involving multiple pathways including protein biosyntheses, glycolysis, TCA cycle, the metabolisms of choline amino acids, fatty acids, purines, and pyrimidines. Most hemolymph amino acids had two elevations during the feeding period of the fourth instar and prepupa stage. Trehalose was the major blood sugar before day 8 of the fifth instar, whereas glucose became the major blood sugar after spinning. C16:0, C18:0 and its unsaturated forms were dominant fatty acids in hemolymph. The developmental changes of hemolymph metabonome were associated with dietary nutrient intakes, biosyntheses of cell membrane, pigments, proteins, and energy metabolism. These findings offered essential biochemistry information in terms of the dynamic metabolic changes during silkworm development.