Cosmid clones derived from both euchromatic and heterochromatic regions of the human Y chromosome.
Cosmid clones derived from both euchromatic and heterochromatic regions of the human Y chromosome.
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来自人类 Y 染色体常染色质和异染色质区域的粘粒克隆。
DOI：
10.1002/j.1460-2075.1984.tb02081.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
P. Goodfellow
中科院分区：
文献类型：
--
作者：
J. Wolfe;R. Erickson;P. Rigby;P. Goodfellow
从人-鼠杂交细胞系的粘粒文库中分离出包含来自人类Y染色体的序列的克隆。这些文库被构建在新的表达载体Hmer V和Hmer VI中。分离到的粘粒集合丰富了独特的序列DNA，只有少数粘粒包含构成Y染色体主要部分的重复序列。对三个宇宙进行了详细的研究。一个粘粒显示出与X染色体长臂至少20kb的广泛同源性；这种同源性在预测的性染色体配对所需的同源性区域之外。另外两个克隆包含Y染色体特有的独特序列，它们都映射到Y染色体长臂的异染色质区域。
Clones containing sequences derived from the human Y chromosome have been isolated from cosmid libraries of a human‐mouse hybrid cell line. These libraries were constructed in the new expression vectors Homer V and Homer VI. The collection of cosmids isolated is enriched for unique sequence DNA and only a few of the cosmids contain the tandemly repeated sequences which constitute a major portion of the Y chromosome. Three cosmids have been studied in detail. One cosmid shows extensive homology over at least 20 kb with the long arm of the X chromosome; this homology is outside the predicted homology region required for sex chromosome pairing. The other two clones contain unique sequences specific to the Y chromosome and both map to the heterochromatic region of the Y chromosome long arm.