Migratory interaction of amphibian epidermal cells with components of the basement membrane.
Migratory interaction of amphibian epidermal cells with components of the basement membrane.
复制标题
两栖动物表皮细胞与基底膜成分的迁移相互作用。
DOI：
10.1002/jcp.1041580111
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发表时间：
1994
影响因子：
5.6
通讯作者：
Chung,AE
中科院分区：
文献类型：
--
作者：
Donaldson,DJ;Mahan,JT;Tsilibary,EC;McCarthy,JB;Dixit,SN;Chung,AE
在成年蝾螈中，新鲜伤口附近的基底表皮细胞通过表皮基底膜迁移到受损区域。为了确定哪些基底膜成分介导这种迁移，将涂有各种天然基质、纯化蛋白质或蛋白质片段的小玻璃片植入皮肤伤口中，以便试图形成伤口上皮的表皮细胞会遇到植入物。源自不产生 IV 型胶原的细胞系 (M1536-B3) 的层粘连蛋白作为迁移底物没有活性。重组巢蛋白上的迁移稍好于层粘连蛋白上的迁移，但仍仅为 I 型胶原上迁移的约 14%。 M15 基质是 M1536-B3 细胞的含有层粘连蛋白和巢蛋白的产物，并不比单独的巢蛋白更好。 IV 型胶原蛋白是一种极好的底物，在几乎所有测试浓度下，产生的迁移略多于相应浓度的 I 型胶原蛋白。缺乏 NC1 结构域的 IV 型的迁移至少与完整的 IV 型一样好。 IV 型的所有活性都存在于 α1 链羧基末端三分之二的 95 kD 片段 (al (IV)95) 中。在涂有源自 α1(IV)95 羧基末端一半的 α1 链 110 个氨基酸片段的植入物上，获得了大约 60% 的 β1(IV)95 活性。在培养基中添加合成肽arg-gly-asp-ser (RGDS)，会阻碍纤连蛋白涂层植入物上的迁移，但对 IV 型涂层植入物没有影响，这表明 IV 型上的迁移涉及与纤连蛋白介导迁移的细胞表面受体不同的细胞表面受体。基质胶是一种含有大部分基底膜成分的商业产品，是一种较差的迁移基质。因此，如果 IV 型体内介导基底细胞向伤口迁移，则基底膜结构可能必须发生一些改变，以允许表皮受体进入 IV 型活性位点。 © 1994 Wiley-Liss, Inc.
In adult newts, basal epidermal cells adjacent to a fresh wound move toward the damaged area by migrating over the epidermal basement membrane. In an attempt to determine which basement membrane components mediate this migration, small pieces of glass coated with various natural matrices, purified proteins, or fragments of proteins were implanted into skin wounds such that epidermal cells attempting to form a wound epithelium would encounter the implants. Laminin derived from a cell line (M1536‐B3) that produces no type IV collagen was inactive as a migration substrate. Migration on recombinant entactin was somewhat better than on laminin but was still only ∼ 14% of that on type I collagen. M15 matrix, a laminin and entactin‐containing product of M1536‐B3 cells, was no better than entactin alone. Type IV collagen was an excellent substrate, producing slightly more migration than corresponding concentrations of type I collagen at nearly all concentrations tested. Migration on type IV lacking the NC1 domain was at least as good as on intact type IV. All the activity in type IV was present in a 95 kD fragment (al (IV)95) from the carboxy terminal two‐thirds of the α1 chain. Approximately 60% of the activity on β1(IV)95 was obtained on implants coated with a 110 amino acid fragment of the α1 chain derived from the carboxy terminal half of α1(IV)95. Adding the synthetic peptide, arg‐gly‐asp‐ser (RGDS) to the medium, biocked migration on fibronectin‐coated implants but had no effect on implants coated with type IV, suggesting that migration on type IV involves different cell surface receptors than those mediating migration over fibronectin. Matrigel, a commercial product containing most basement membrane components, was a poor migration substrate. Thus if type IV mediates basal cell migration toward a wound in vivo, there may have to be some alterations in basement membrane structure to allow epidermal receptors to access type IV active site(s). © 1994 Wiley‐Liss, Inc.