Mammalian nitrate biosynthesis: incorporation of 15NH3 into nitrate is enhanced by endotoxin treatment.
Mammalian nitrate biosynthesis: incorporation of 15NH3 into nitrate is enhanced by endotoxin treatment.
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哺乳动物硝酸盐生物合成：内毒素处理可增强 15NH3 与硝酸盐的结合。
DOI：
10.1073/pnas.80.14.4518
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发表时间：
1983
影响因子：
11.1
通讯作者：
Tannenbaum,SR
中科院分区：
文献类型：
--
作者：
Wagner,DA;Young,VR;Tannenbaum,SR
口服剂量的[15N]醋酸铵加入到大鼠的尿[15N]硝酸盐中。对硝酸盐合成调节的研究表明，大肠杆菌脂多糖能有效刺激尿中硝酸盐的排泄(增加9倍)。进一步证明，内毒素促进硝酸盐排泄不是由于硝酸盐代谢损失的减少，而是由于合成速度的增加。这一结论是基于通过内毒素处理发现硝酸盐氮中[15N]铵的比例增加。腹腔注射卡拉胶和皮下注射松节油也能促进硝酸盐的生物合成。有人认为，硝酸盐生物合成的途径可能是由激活的网状内皮系统产生的氧自由基氧化还原的氮化合物的结果。
Incorporation of an oral dose of [15N]ammonium acetate into urinary [15N]nitrate has been demonstrated in the rat. Investigation of the regulation of nitrate synthesis has shown that Escherichia coli lipopolysaccharide potently stimulates urinary nitrate excretion (9-fold increase). It was further shown that the enhanced rate of nitrate excretion by lipopolysaccharide was due not to a reduction in nitrate metabolic loss but rather to an increased rate of synthesis. This conclusion was based on finding a proportionally increased incorporation of [15N]ammonium into nitrate nitrogen with lipopolysaccharide treatment. Nitrate biosynthesis was also increased by intraperitoneal injection of carrageenan and subcutaneous injection of turpentine. It is proposed that the pathway of nitrate biosynthesis may be the result of oxidation of reduced nitrogen compounds by oxygen radicals generated by an activated reticuloendothelial system.