Evaluation of Serum Testosterone, Progesterone, Seminal Antisperm Antibody, and Fructose Levels among Jordanian Males with a History of Infertility.
Evaluation of Serum Testosterone, Progesterone, Seminal Antisperm Antibody, and Fructose Levels among Jordanian Males with a History of Infertility.
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DOI：
10.1155/2010/409640
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发表时间：
2010
影响因子：
3
通讯作者：
Abu Zaid M
中科院分区：
文献类型：
--
作者：
Al-Daghistani HI;Hamad AW;Abdel-Dayem M;Al-Swaifi M;Abu Zaid M
由于精液的生化复杂性，我们试图研究在雄激素作用下分泌的果糖与自身免疫之间可能的相关性，而自身免疫可能在精索静脉曲张相关的不孕症和精子活力降低方面发挥作用。研究人员测量了 66 名患有精索静脉曲张的不育男性和 84 名转诊接受生育治疗的无精索静脉曲张的不育男性的精液果糖、抗精子抗体 (ASA) 和血液类固醇激素（睾酮和黄体酮）水平。通过精液果糖的生化测量和 ASA 的混合凝集反应 (MAR) 进行精液分析。使用竞争性化学发光酶免疫测定法评估孕酮和睾酮的血清水平。血清睾酮平均值为 380.74 ± 24.331、365.9 ± 16.55 和 367.5 ± 21.8 ng/dl，孕酮平均值为 0.325 ± 0.243、0.341 ± 0.022 和 0.357 ± 0.0306 ng/ml，精液平均值为精索静脉曲张男性、未精索静脉曲张男性和生育男性的血浆果糖分别为 359.6 ± 26.75、315.6 ± 13.08 和 332.08 ± 24.38 mg/dl。在精索静脉曲张组中观察到睾酮水平显着升高 (P = .001)。该结果表明，睾酮可能是精索静脉曲张受试者不育的决定因素。在 18 例（26.47%）精索静脉曲张病例、20 例（38.46%）无精索静脉曲张病例和 16 例（32.0%）育龄男性中检测到 ASA。 ASA 病例与精子活力低下形态相关。精子结合抗体和粘度之间已显示出负相关 (P = .017)。 ASA 与年龄、不孕持续时间和粘度存在显着的负相关关系 (P < .05)。此外，在不育病例（有或没有精索静脉曲张）和可育病例中，ASA 阳性精浆和睾酮浓度之间观察到显着相关性（P < .05）。我们的研究结果表明，睾丸类固醇激素水平与自身免疫和精子抗体之间存在关系，精子抗体影响约旦不育男性射精精子的活力。
Due to the biochemical complexity of seminal fluid, we attempt to study the possible correlation between fructose, which is secreted under the effect of androgen hormone, and autoimmunity, which might play a role in varicocele associated infertility, in reducing sperm motility. Seminal fructose, antisperm antibodies (ASAs) and blood steroids hormones (testosterone and progesterone) levels were measured in 66 infertile males with varicocele and 84 without varicocele referred for fertility treatment. Seminal analysis was performed with biochemical measurements of seminal fructose and mixed agglutination reaction (MAR) for ASA. Serum levels of progesterone and testosterone were estimated using a competitive chemoluminescent enzyme immunoassay. The mean values for serum testosterone were 380.74 ± 24.331, 365.9 ± 16.55, and 367.5 ± 21.8 ng/dl, progesterone 0.325 ± 0.243, 0.341 ± 0.022, and 0.357 ± 0.0306 ng/ml, and seminal plasma fructose 359.6 ± 26.75, 315.6 ± 13.08, and 332.08 ± 24.38 mg/dl in males with varicocele, without varicocele, and fertile males, respectively. A significant high level of testosterone was observed within varicocele group (P = .001). This result showed that testosterone may play a role as an infertility determinant in subjects with varicocele. ASA was detected in 18 (26.47%) of cases with varicocele, 20 (38.46%) without varicocele, and in 16 (32.0%) fertile men. Cases with ASAs associated with low sperm motility morphology. An inverse correlation between sperm-bound antibodies and viscosity has been shown (P = .017). ASA showed some significant inverse relations with ages, durations of infertility, and viscosity (P < .05). In addition, a significant correlation was observed between ASA positive seminal plasma and testosterone concentration among infertile cases (with or without varicocele) and fertile (P < .05). Our results suggest a relationship between testicular steroid hormone levels with autoimmunity and sperm antibodies which influence the motility of ejaculated spermatozoa among Jordanian infertile males.