Ependymal epithelium disruption after Vanadium pentoxide inhalation -: A mice experimental model
Ependymal epithelium disruption after Vanadium pentoxide inhalation -: A mice experimental model
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DOI：
10.1016/j.neulet.2005.01.072
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发表时间：
2005-06-10
影响因子：
2.5
通讯作者：
Fortoul, TI
中科院分区：
文献类型：
--
作者：
Avila-Costa, MR;Colín-Barenque, L;Fortoul, TI
血脑屏障（BBB）保护CNS免受化学损伤。血脑组织交换的调节是由具有细胞间紧密连接的室管膜细胞完成的。BBB功能丧失是许多神经系统疾病的病因组成部分。钒（V）是一种广泛分布于环境中的类金属，对多种生物系统具有潜在的毒性作用。本研究通过对小鼠吸入五氧化二钒（V2O5）8周后脑内金属离子浓度的变化及扫描电镜和透射电镜下室管膜细胞形态学改变的分析，探讨V2O5进入中枢神经系统的可能机制。我们的研究结果表明，V2O5浓度从研究的第一周开始增加，在实验的其余部分稳定其值。形态学改变包括纤毛脱落、细胞脱落和室管膜细胞层脱离。这种损伤可以允许毒物改变上皮的渗透性，并促进炎症介质进入下层神经元组织，导致损伤和神经元死亡。因此。了解血脑屏障破坏的机制将允许规划策略以保护大脑免受有毒物质如金属的影响，这些有毒物质在过去几十年中在大气中增加并构成重要的健康问题。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
The blood-brain barrier (BBB) protects the CNS against chemical insults. Regulation of blood-brain tissue exchange is accomplished by ependymal cells, which possess intercellular tight junctions. Loss of BBB function is an etiologic component of many neurological disorders. Vanadium (V) is a metalloid widely distributed in the environment and exerts potent toxic effects on a wide variety of biological systems. The current study examines the effects of Vanadium pentoxide (V2O5) inhalation in mice ependymal epithelium, through the analysis of the brain metal concentrations and the morphological modifications in the ependymal cells identified by scanning and transmission electron microscopy after 8 weeks of inhalation, in order to obtain a possible explanation about the mechanisms that V uses to enter and alter the CNS. Our results showed that V2O5 concentrations increase from the first week of study, stabilizing its values during the rest of the experiment. The morphological effects included cilia loss, cell sloughing and ependymal cell layer detachment. This damage can allow toxicants to modify the permeability of the epithelium and promote access of inflammatory mediators to the underlying neuronal tissue causing injury and neuronal death. Thus. understanding the mechanisms of BBB disruption would allow planning strategies to protect the brain from toxicants such as metals, which have increased in the atmosphere during the last decades and constitute an important health problem. (c) 2005 Elsevier Ireland Ltd. All rights reserved.