Ultrasound-mediated disruption of cell membranes.: II.: Heterogeneous effects on cells
Ultrasound-mediated disruption of cell membranes.: II.: Heterogeneous effects on cells
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DOI：
10.1121/1.1376130
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发表时间：
2001-07-01
影响因子：
2.4
通讯作者：
Prausnitz, MR
中科院分区：
文献类型：
--
作者：
Guzmán, HR;Nguyen, DX;Prausnitz, MR
超声波已被证明通过一种被认为涉及空化的机制可逆地和不可逆转地破坏活细胞的膜。由于空化在时间和空间上都是不同的，因此使用流式细胞术来鉴定和量化超声对分子摄取和细胞存活率影响的异质性，以细胞为基础，将DU145前列腺癌和主动脉平滑肌细胞悬液暴露在不同的峰值负声压(0.6-3.0兆帕)、暴露时间(120-2000毫秒)和脉冲长度(0.02-60毫秒)的Optison(1.7%v/v)造影剂存在下。在所研究的所有条件下观察到细胞间的异质性，并将其分为三个亚群：标称摄取(NUP)、低摄取(LUP)和高摄取(HUP)。每个亚群的平均分子数基本恒定：NUP为10(4)-10(5)个分子/细胞，与LUP的10(6)个分子/细胞相似，与HUP的10(7)个分子/细胞相似。然而，每个亚群中的细胞比例显示出对声压和暴露时间的强烈依赖。改变脉冲长度不会产生明显的影响。细胞在三个亚群中的分布与声能暴露有关，这表明能量暴露可能通过非热机制控制超声诱导生物效应的能力。(C)2001年美国声学学会。
Ultrasound has been shown to reversibly and irreversibly disrupt membranes of viable cells through a mechanism believed to involve cavitation. Because cavitation is both temporally and spatially heterogeneous, flow cytometry was used to identify and quantify heterogeneity in the effects of ultrasound on molecular uptake and cell viability on a cell-by-cell basis for suspensions of DU145 prostate cancer and aortic smooth muscle cells exposed to varying peak negative acoustic pressures (0.6-3.0 MPa), exposure times (120-2000 ms), and pulse lengths (0.02-60 ms) in the presence of Optison (1.7% v/v) contrast agent. Cell-to-cell heterogeneity was observed at all conditions studied and was classified into three subpopulations: nominal uptake (NUP), low uptake (LUP), and high uptake (HUP) populations. The average number of molecules within each subpopulation was generally constant: 10(4)-10(5) molecules/cell in NUP, similar to 10(6) molecules/cell in LUP, and similar to 10(7) molecules/cell in HUP. However, the fraction of cells within each subpopulation showed a strong dependence on both acoustic pressure and exposure time. Varying pulse length produced no significant effect. The distribution of cells among the three subpopulations correlated with acoustic energy exposure, which suggests that energy exposure may govern the ability of ultrasound to induce bioeffects by a nonthermal mechanism. (C) 2001 Acoustical Society of America.