Biocompatibility of Tygon® tubing in microfluidic cell culture.
Biocompatibility of Tygon® tubing in microfluidic cell culture.
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Tygon® 管在微流体细胞培养中的生物相容性。
DOI：
10.1007/s10544-015-9938-9
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发表时间：
2015
影响因子：
2.8
通讯作者：
Gamcsik,MichaelP
中科院分区：
文献类型：
--
作者：
Jiang,Xiao;Jeffries,RexE;Acosta,MiguelA;Tikunov,AndreyP;Macdonald,JeffreyM;Walker,GlennM;Gamcsik,MichaelP
当培养基通过微孔 Tygon® 管输送至通道时，微流体通道中的 MDA-MB-231 乳腺癌细胞系的生长受到抑制。在该管内孵育的培养基也抑制了这些细胞在传统 96 孔板中的生长。这些有害影响并不是由于介质成分吸附到管道表面而导致关键营养物质的消耗。 pH 值变化也被排除为原因。在管中孵育之前和之后对细胞生长培养基的核磁共振光谱证实没有可检测到的培养基成分损失，但确实检测到在光谱的脂肪族区域中存在额外的未识别信号。这些结果表明从微孔 Tygon® 管中浸出的化学物质会影响微流体装置中的细胞生长。
Growth of the MDA-MB-231 breast cancer cell line in microfluidic channels was inhibited when culture media was delivered to the channels via microbore Tygon® tubing. Culture media incubated within this tubing also inhibited growth of these cells in conventional 96-well plates. These detrimental effects were not due to depletion of critical nutrients due to adsorption of media components onto the tubing surface. A pH change was also ruled out as a cause. Nuclear magnetic resonance spectroscopy of the cell growth media before and after incubation in the tubing confirmed no detectable loss of media components but did detect the presence of additional unidentified signals in the aliphatic region of the spectrum. These results indicate leaching of a chemical species from microbore Tygon® tubing that can affect cell growth in microfluidic devices.