Fabrication of photoluminescent hybrid protein crystals of lysozyme and bromophenol blue
Fabrication of photoluminescent hybrid protein crystals of lysozyme and bromophenol blue
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DOI：
10.1007/s10853-015-9255-y
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发表时间：
2015-07
影响因子：
4.5
通讯作者：
Mina Guli;Mei Li;Zhe Hu;Jianxi Yao;S. Mann
中科院分区：
文献类型：
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作者：
Mina Guli;Mei Li;Zhe Hu;Jianxi Yao;S. Mann
通过将溴酚蓝染料分子掺入交联溶菌酶单晶的有组织溶剂通道中，获得了表现出增强的光致发光的混合蛋白质晶体。从FT-IR，TEM，EDX，UV-Vis，和共聚焦表征，我们表明，BpB染料是内部的溶剂通道的晶体已绑定的Trp 62和Trp 108残基的蛋白质。
Hybrid protein crystals exhibiting enhanced photoluminescence were obtained by incorporation of bromophenol blue dye molecules within the organized solvent channels of cross-linked lysozyme single crystals. From the FT-IR, TEM, EDX, UV–Vis, and confocal characterization, we show that the BpB dye is inside the solvent channels of the crystals which has been bound up with the Trp62 and Trp108 residues of the protein.